黄芩苷对黑质内注射三氯化铁所致中脑损毁的保护作用

目的 探讨黑质部位注射FeCl_3制备帕金森病模型的可行性,并观察黄芩苷的保护作用.方法 雄性Wistar大鼠,黄芩苷组术前灌胃黄芩苷(78 mg·kg~(-1)·d~(-1))1周;模型组和黄芩苷组均单侧黑质定向注射FeCl_3(40μg)造摸,术后第5周中脑黑质病理切片行免疫组化分析酪氨酸羟化酶(TH);铁染色观察中脑黑质铁含量.结果 ①造模后2～3 d,动物头、身体向注射对侧倾斜,部分动物自发向注射对侧旋转,有些伴有抽搐和强直.术后7 d尚见有不同程度类帕金森病表现,震颤、弓背;黄芩苷组死亡率(模型组46%,黄芩苷组36%)降低.②注射侧中脑见明显的铁沉积,脑组织损毁严重.而黄芩苷组未见这一现象.③注射侧黑质多巴胺能神经细胞明显缺失.黄芩苷预防给药1周没能阻止这种损伤.结论 铁剂黑质注射造成的中脑损毁严重,动物有类帕金森病表现.黄芩苷能阻止铁在中脑沉积及所造成的损毁,显示明显的保护作用.

The Protective Effect of Baicalin against the Degeneration of Nigral Dopaminergic Neurons and Lesion of the Mesencephalon Induced by FeCl3 in Rats

Objective To explore the feasibility of developing Parkinson's disease model induced by injecting FeCl_3 into nigrostriatum, and to observe the protective effect of baicalin on the model.Methods The Wistar rats of drug-group were administered baicalin (78mg·kg~(-1)·d~(-1)) by oral for 1 week. The FeCl_3 (40μg) was injected into the unilateral substantia nigra (SN) on all rats. The dopamine neurons at the mesencephalon nigra were observed by tyrosine hydroxylase (TH) immunohistochemistry in the 5th week after injection. The iron contents were measured by iron stain. Results ①The head and body of animals was gradient toward side of the opposite injected mesencephalon after operated for 2-3 days, spontaneous rotation behaviors toward the opposite side of injected side and convuision and tetany for some rats. Some similar Parkinson's disease behavior appeared, such as thrill, hunchback on the 7th day after injection, mortality were reduced in rats of Baicalin-group rats. ②Iron deposit in the nigrostriatum and lesions of the mesencephalon appeared on injected side in the model-group, but it weren't in rats of Baicalin-group. ③The Nigrostriatal dopaminergic neurons were decreased on injected side and this decrease could not be prevented by Baicalin treatment prior 1 week.Conclusion The lesion of the mesencephalon induced by FeCl_3 injection is severe, and some similar Parkinson's disease behaviors can appeare.The results we report here offered support that the protective effect of baicalin on Iron gather and mesencephlalon lesion induced FeCl_3 injection in rats.