胃癌多药耐药细胞药物积累的异常

Abnormal drug accumulation in multidrug resistant gastric carcinoma cells

AIM To study the difference in intracellular concentration of drug between multidrug resistant cells and drug sensitive cells of gastric carcinoma. METHODS Multidrug resistant gastric carcinoma cell sublines were established by a stepwise increase of concentration of vincristine in media. The uptake of drug was measured by isotope liquid scintillation counter. Laser confocus microscope was used to study the intracellular distribution of daunorubicin and fluorescent intensity of daunorubicin in drug- resistant and drugsensitive cells after incubated in drug-free media for various time periods. RESULTS Fluorescent of daunorubicin was mainly contained in the nuclei of both sensitive and resistant cells. The fluorescent intensity of daunorubicin in drugresistant cells was lower after incubated in drug-free media for various time periods. CONCLUSION The multidrug resistant gastric cells present a lower rate of drug uptake, and the rate of efflux drug was greater than the sensitive parent cell lines, resulting in the decrease of the intracellular concentration of anticancer drug in multidrug resistant calls, and the cytotoxity of drug.