抗MPO抗体的ELISA检测和应用

本文应用Ｍａｔｈｅｓｏｎ方法从人白细胞中提取髓过氧化物酶ＭＰＯ，建立ＥＬＩＳＡ法检测６３份血清标本中抗ＭＰＯ抗体，同时与间接免疫荧光法（ＩＩＦ）作比较，结果是：１９例结节性多动脉炎（ＰＡＮ）患者中６例抗ＭＰＯ抗体阳性（３２％），较ＩＩＦ法阳性率提高（１９例中１例），而２２例正常人及２２例其他肾脏病患者血清均为阴性。提示ＥＬＩＳＡ法既可从分子水平鉴定系统性血管炎致病的特异性抗体，又是系统性血管炎敏感的诊断方法。

Detection of Antimyeloperosidase Antibodies by ELISA

A specific enzyme-linked immunosorbent assay (ELISA) for detection of antimyeloperoxidase (MPO) antibodies was established. Human MPO was obtained by Matheson’s method, which include dialysis of a granucle extract against low salt buffer, sephadex G-75 chromatography, and carboxymethylcellulos chromatography.Using established ELISA method, 6 of 19 patients with polyarteritis nodosa (PAN)were found to be positive, while only 1 positive by IIF. No positive was found in the normal control and patients with other renal diseases. The results suggested ELISA was a sensitive, specific and reproducible method for detection of antimyeloperoxidase antibodies.