Taqman荧光探针技术检测结核分枝杆菌

目的：比较Taqman荧光探针技术与常规PCR电泳技术检测结核分枝杆菌的灵敏度及特异性，评价Taqman荧光探针技术（Taqman技术）检测临床标本中结菌的应用价值。方法：应用细菌培养法，常规PCR电泳技术与Taqman技术检测300份临床结核病患者痰标本及50份非结核呼吸系统疾病患者痰标本。结果：细菌培养法检测结核阳性率为112/300；常规PCR电泳技术检测结核菌阳性率为169/300，特异性96%；Taqman荧光探针技术检测结核菌阳性率为141/300，特异性100％。结论：Taqman荧光探针技术是一种全新的PCR分子杂交模式，集PCR扩增、荧光探针杂交、荧光检测一体化，在单一管内完成，简便、快速、防污染、敏感性高、特异性强，是结核病辅助诊断的有效方法。

TAQMAN PCR FLUORESCENCE PROBE TECHNIQUE IN DETECTING MYCOBACTERIUM TUBERCULOSIS

Objective:To compare the sensitivity and specificity of Taqman fluorescence probe technique and routine PCR electrophoresis technique,and evaluate the application value of Taqman technique in detecting clinical mycobacterium tuberculosis.Methods:Three hundred sputum samples of tubercular and 50 sputum samples of nontubercular respiratory system disease were detected by bacillary culture method,roution PCR and Taqman technique.Results:The positive rates of culture method,routine PCR and Taqman technique were 112/130,169/300 and 141/300 respectively.The specificity of routine PCR and Taqman technique were 96% and 100% respectively.Conclusion:Taqman technique is a new PCR molecular hybridization model,it is simple,rapid and contaminateproof,and shows a high specificity annd sensitivity.It is an effective method for diagnosing tuberculosis (TB).