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Immune Profiling of T Cells Infiltrating Vitreous Humor in Tubercular Uveitis |
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| Authors: | Ravi Kumar Sharma M.Sc Vishali Gupta M.S Reema Bansal M.S Kusum Sharma M.D Amod Gupta M.S |
| Affiliation: | 1. Department of Ophthalmology, Advanced Eye Centre, Post Graduate Institute of Medical Education and Research, Chandigarh, India;2. Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India |
| Abstract: | Purpose: To assess cellular composition and local cytokine response in vitreous humor of tubercular uveitis. Methods: Cells were collected from vitreous cassettes and peripheral blood of 8 tubercular uveitis and 5 control subjects, undergoing vitrectomy and analyzed by flow cytometry for cellular composition, activation status, proinflammatory cytokine expression, and uptake of TLR9 ligand, CpG ODN 2216. Results: CD3 + T cells with equal proportion of CD4+ and CD8 + T cells formed major fraction of infiltrating cells. The vitreous humor showed higher expression of recent activation marker, CD69, and proinflammatory cytokines, IFN-γ and IL-17A, in CD4 + T cells as compared to peripheral blood. Lastly, intraocular CD4 + T cells showed reduced uptake of ODN 2216 than peripheral blood. Conclusions: Our results indicate that local antigenic stimuli trigger T cell infiltration and activation of CD4 + T cells that are hyporesponsive to TLR9 stimulation. These infiltrating T cells might be responsible in further aggravating ocular inflammation. |
| Keywords: | Activated T cell T cells Th1 response Th17 response TLR9 Tubercular uveitis Vitreous humor |