RIPK4在骨肉瘤组织和细胞中的表达及其意义

目的研究RIPK4（Receptor-interacting protein kinase 4）基因在骨肉瘤组织及骨肉瘤细胞中的表达情况并探讨其表达的临床意义。方法 采用免疫组化染色法检测30例骨肉瘤及10例骨软骨瘤患者瘤组织中RIPK4表达情况，分析其表达与骨肉瘤临床病理特征的关系；RT-PCR检测正常成骨细胞、骨肉瘤MG-63及U-2OS细胞中RIPK4 mRNA表达情况，并比较其差异。结果 30例骨肉瘤中RIPK4阳性表达18例（60.0％），10例骨软骨瘤中RIPK4阳性表达2例（20.0％），差异有统计学意义（P＜0.05）。正常成骨细胞中RIPK4 mRNA为0.6500±0.2121，低于骨肉瘤MG-63细胞的1.6079±0.2661及U-2OS细胞的1.5000±0.1414，差异有统计学意义（P＜0.05）。RIPK4蛋白表达与临床分期及是否发生肺转移显著相关，差异有统计学意义（P＜0.05），而与性别、年龄及病理分型等无关（P＞0.05）。结论 RIPK4 mRNA和蛋白在骨肉瘤组织及细胞中均有较强表达，RIPK4基因可能参与骨肉瘤的发生发展过程。

The expression of RIPK4 in osteosarcoma tissues and cells and its clinical significance

Objective To investigate the expression and significance of RIPK4 protein in human osteosarcomatous tissue and cells. Methods Imunohistochemistry was performed to detect the expression of RIPK4 protein in paraffin speciments of 30 osteosarcoma patients（and 10 osteochondroma patients. The correlation among clinicopathologic characteristics and RIPK4 protein was analyzed. The expression of RIPK4 mRNA in osteosarcoma MG-63， U-2OS and osteoblast cells was detected by RT-PCR， and differences were compared. Results The positive expression of RIPK4 in osteosarcoma was 60.0％（18/30）， while it was 20.0％（2/10） in osteochondroma.The differences were statistically significant（P＜0.05）. RIPK4 mRNA of osteoblast cells was 0.6500±0.2121， which was significantly lower than that of MG-63（1.6079±0.2661） and U-2OS（1.5000±0.1414），with singnificant differences（P＜0.05）. The expression of RIPK4 protein was related to TNM stage and lung metastasis（P＜0.05）. Conclusion The expression of RIPK4 protein and gene in osteosarcoma tissues and cells is high. RIPK4 may be involved in the progress of epithelial osteosarcoma.