塞来昔布减轻糖尿病大鼠心肌细胞纤维化的实验研究

目的 探讨塞来昔布对糖尿病心肌病大鼠心肌细胞纤维化的影响.方法 将Wistar大鼠30只随机分为对照组（A组）、糖尿病心肌病组（B组）和糖尿病心肌病塞来昔布治疗组（C组）各10只.取大鼠心肌组织,经常规石蜡切片、染色后测定胶原纤维面的密度值;采用RT-PCR法测定TGF-β1 mRNA表达情况.结果与A组相比,B组大鼠心肌组织的胶原纤维密度值、TGF-β1 mRNA表达明显升高（P〈0.05）.C组大鼠的胶原纤维、TGF-β1 mRNA表达明显低于A组和B组（均P〈0.05）.结论塞来昔布可能通过抑制TGF-β1减轻糖尿病大鼠心肌纤维化.

Experimental study of celecoxib alleviating diabetic myocardial fibrosis in rats

Objective To investigate the effect of celecoxib on myocardial fibrosis in diabetic rats. Methods Wistar rats were randomly divided into normal control group （A group, n= 10）, diabetic myocardial group （B group, n= 10） and diabetic cardiomyopathy celecoxib treatment group （C group, n= 10）. The rats were sacrificed and myocardial tissues underwent paraffin and staining tissue sections, the surface density of collagen fibers level was detected. The expression of transforming growth factor-βl （TGF-β1） mRNA was detected by RT-PCR. Results Compared with the normal control group, the collagen fibers of myocardial tissue in diabetic rats, TGF-β1 mRNA expression was significantly increased （P〈0. 05）. The collagen fibers in celecoxib treated rats, TGF-β1 mRNA expression was significantly lower than the diabetic myocardial group and normal control group （P〈0. 05）. Conclusion Celecoxib can alleviate development of myocardial fibrosis in diabetic rats.