| 藤三七总皂苷和总黄酮提取工艺优选 |
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| 引用本文： | 蒋珍藕,邱宏聪,邓聿胤.藤三七总皂苷和总黄酮提取工艺优选[J].中国实验方剂学杂志,2013,19(19):28-30. |
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| 作者姓名： | 蒋珍藕 邱宏聪 邓聿胤 |
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| 作者单位： | 广西中医药研究院, 南宁 530022;广西中医药研究院, 南宁 530022;广西中药质量标准研究重点实验室, 南宁 530022;广西中医药研究院, 南宁 530022 |
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| 基金项目： | 广西医疗卫生重点科研课题(重2010112) |
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| 摘 要： | 目的: 优选藤三七总皂苷和总黄酮的提取工艺。方法: 采用UV测定总皂苷和总黄酮含量。以总皂苷和总黄酮提取量为指标,采用单因素试验考察提取次数,正交试验考察乙醇体积分数、提取时间及乙醇用量对藤三七中总皂苷和总黄酮提取工艺的影响。结果: 藤三七中总皂苷和总黄酮的最佳提取工艺为加12倍量75%乙醇回流提取2次,每次2 h,总皂苷、总黄酮平均提取量分别为16.23,46.40 mg·g-1。结论: 优选的提取工艺合理可行。
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| 关 键 词： | 藤三七 总皂苷 总黄酮 提取工艺 |
| 收稿时间： | 2013/5/24 0:00:00 |
Optimization of Extraction Technology of Total Saponins and Total Flavonoids from Anredera cordifolia |
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| JIANG Zhen-ou,QIU Hong-cong and DENG Yu-yin.Optimization of Extraction Technology of Total Saponins and Total Flavonoids from Anredera cordifolia[J].China Journal of Experimental Traditional Medical Formulae,2013,19(19):28-30. |
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| Authors: | JIANG Zhen-ou QIU Hong-cong and DENG Yu-yin |
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| Institution: | Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China;Guangxi Key Laboratory of Quality Standards of Traditional Chinese Medicine, Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Science, Nanning 530022, China |
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| Abstract: | Objective: To optimize extraction technology of total saponins and total flavonoids from Anredera cordifolia. Method: The contents of total saponins and total flavonoids were determined by UV.With extraction amounts of total saponins and total flavonoids as indexes,extraction times was investigated by single factor test,effects of ethanol concentration,extraction time and ethanol amount on extraction technology of total saponins and total flavonoids were investigated by orthogonal design. Result: The best extraction conditions for total saponins and total flavonoids from A. cordifolia was as following:extracted 2 times with 12-fold the amount of 75% ethanol,2 h per time;Average extraction volume of total saponins and total flavonoids were 16.23,46.40 mg·g-1,respectively. Conclusion: Optimized extraction process was reasonable and feasible. |
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| Keywords: | Anredera cordifolia total saponins total flavonoids extraction technology |
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