| 千层纸素A在Caco-2细胞模型中的吸收机制研究 |
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| 引用本文： | 冯志强,谢智勇,廖琼峰,姚美村,马燕,张蕾.千层纸素A在Caco-2细胞模型中的吸收机制研究[J].中南药学,2011,9(7):481-485. |
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| 作者姓名： | 冯志强 谢智勇 廖琼峰 姚美村 马燕 张蕾 |
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| 作者单位： | 1. 中山大学药学院,广州,510006
2. 广州中医药大学,广州,510006 |
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| 基金项目： | 国家自然科学基金项目，国家教育部新教师基金项目，“重大新药创制”国家科技重大专项项目 |
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| 摘 要： | 目的研究千层纸素A在Caco-2细胞模型中的吸收机制。方法 MTT实验考察千层纸素A在Caco-2细胞中的安全浓度范围,再利用Caco-2细胞单层模型研究千层纸素A的双向转运机制,以转运量及表观渗透系数(Papp)为指标,考察时间、浓度、pH和P-gp抑制药维拉帕米对其吸收的影响。结果千层纸素A在Caco-2细胞模型中的转运与时间和浓度呈正相关;并受pH值影响,P-gp抑制药维拉帕米对其转运无影响,从单层细胞层顶端(AP)到基底端(BL)的转运与基底端到顶端的转运大致相同。结论千层纸素A在Caco-2细胞模型中的吸收是被动转运。
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| 关 键 词： | 千层纸素A Caco-2细胞模型 高效液相色谱 被动转运 |
Absorption of oroxylin A across Caco-2 cell monolayer model |
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| FENG Zhi-qiang,XIE Zhi-yong,LIAO Qiong-feng,YAO Mei-cun,MA Yan,ZHANG Lei.Absorption of oroxylin A across Caco-2 cell monolayer model[J].Central South Pharmacy,2011,9(7):481-485. |
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| Authors: | FENG Zhi-qiang XIE Zhi-yong LIAO Qiong-feng YAO Mei-cun MA Yan ZHANG Lei |
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| Institution: | FENG Zhi-qiang1,XIE Zhi-yong1,LIAO Qiong-feng2,YAO Mei-cun1,MA Yan2,ZHANG Lei2*(1.School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,2.Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006) |
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| Abstract: | Objective To investigate the absorption mechanism of oroxylin A across Caco-2 cell monolayer model.Methods The safety concentration of oroxylin A in Caco-2 cell was first selected by MTT method.The Caco-2 cell monolayer drug transport model was assigned to study the bi-direction transport mechanism of oroxylin A by evaluating the influence factors such as time,concentration,pH,and P-gp inhibitor of verapamil on its absorption characterization.Results In the Caco-2 cell monolayer model,the transport volume w... |
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| Keywords: | oroxylin A Caco-2 cell monolayer model HPLC passive transference |
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