经血脑屏障载药纳米粒的研究

目的克服血脑屏障的有效方法,是利用纳米粒作为载体将药物转运到中枢神经,使载药纳米粒通过血脑屏障。方法制作经聚山梨脂(TweenR80)修饰的纳米粒,使其通过血脑屏障。检测小鼠的存活率,跟踪脑内毛细血管内皮细胞的吸收量。结果小鼠的存活率明显的增加(存活率达到6个月的小鼠增加到40%)。结论经聚山梨脂80修饰的阿霉素纳米粒药物对通过血脑屏障有很重大的临床意义。

Study on drug-loaded nanoparticles transferring to bloodbrain barrier(BBB)

Object To deliver nanoparticles through the blood-brain barrier(BBB),which represents an insurmountable obstacle for the delivery of a large number of drugs to the central nervous system(CNS).Methods Produce(TweenR 80)nanoparticles,and make it through the blood-brain barrier.The survival rate of mice was detected,and the capacity was tracked to absorb the brain capillary endothelial cells Results Experiments with the extremely aggressive glioblastoma transplanted intracranially showed a long term survival for 6 months of up to 40% of the rats after intravenous injection of the polysorbate 80-coated nanoparticle preparation.The mechanism of the drug through the blood-brain barrier with the nanoparticles appeared to be endocytotic uptake by brain capillary endothelial cells followed either by release of the drugs in these cells and diffusion into the brain or by transcytosis.Conclusions Polysorbate 80-coated nanoparticles represent a very promising preparation for the delivery of drugs across the blood-brain barrier.