乙型肝炎患者血清粒细胞-巨噬细胞集落刺激因子检测的临床意义

目的：探讨血清粒细胞—巨噬细胞集落刺激因子(GM-CSF)对乙型肝炎患者的临床意义。方法：采用放射免疫法检测各型乙型肝炎和肝炎后肝硬化患者与健康受试者血清GM-CSF浓度，常规检测外用血白细胞(WBC)计数，聚合酶链反应(PCR)法检测外用血乙肝病毒基因组(HBV DNA)含量。结果：与正常人相比，慢性肝炎、肝炎后肝硬化患者血清GM-CSF水平、WBC水平下降(P＜0．05)；重型肝炎患者血清GM-CSF水平显著下降(P＜0．01)，WBC水平显著升高(P＜0．01)。HBV DNA含量在急性肝炎、肝炎后肝硬化、慢性重型肝炎、慢性肝炎中依次升高。血清GM-CSF浓度基本与WBC的计数量变化一致。结论：乙型肝炎患者血清GM-CSF水平对WBC水平的影响强于乙肝病毒，且有抗病毒作用。

Clinical Significance of Granulocyte-macrophage Colony-stimulating Factor in Chron-ic Hepatitis B

Objective: To study the changes of granulocyte - macrophage colony - stimulating factor (GM-CSF), the count of write blood cells and HBV DNA of Pallets with different type of hepatitis B. Methods: Sera obtained from 60 patients in 4 groups respectively with a-cute hepatitis B, chronic hepatitis B, gravity hepatitis B and posthepatitis cirrhosis were examined using GM-CSF kit. The count of WBC and HBV DNA were detected by rutine. 15 healthy donors were used as control. Results: Compared with healthy, HBV DNA level was medium, the concentration of GM-CSF and WBC had no changes in acute hepatitis; HBV DNA was high, the concentration of GM-CSF and WBC decreased obviously in chronic hepatitis and posthepatitis cirrhosis; HBV DNA was high, the concentration of GM - CSF decreased obviously, the level of WBC increased obviously in gravity hepatitis B. The concentration of GM - CSF was parallel to the level of WBC but to HBV DNA. Conclusions: The concentration of GM-CSF can not only affect the count of WBC but also resist the virus.