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| 人成纤维样滑膜细胞冻存时间对其活性的影响 | |
| 引用本文： | 戴杏,郭晓蓉,常艳,魏伟. 人成纤维样滑膜细胞冻存时间对其活性的影响[J]. 中国药理学通报, 2009, 25(6) |
| 作者姓名： | 戴杏 郭晓蓉 常艳 魏伟 |
| 作者单位： | 安徽医科大学临床药理研究所,抗炎免疫药理学省部共建教育部重点实验室,抗炎免疫药物安徽省工程技术研究中心,安徽,合肥,230032 |
| 基金项目： | 国家自然科学基金，安徽省科技攻关计划，高等学校博士学科点专项科研基金，高校首批科技创新团队基金，安徽省研究实验基地优秀中青年科研带头人基金，安徽医科大学科学技术研究基金 |
| 摘 要： | 目的探讨适合人成纤维样滑膜细胞(FLS)低温冷藏保存的最佳冻存时间。方法将FLS经-80℃冰箱冷冻保存。在冷冻后2、3、4、5wk分别复苏,培养。观察FLS的生长情况和细胞存活率及24h贴壁率。结果冻存5wk的FLS细胞存活率(66±1.3)%,较冻存2wk(90±2.0)%有明显下降;冻存5wk的FLS24h贴壁率(61.3±2)%,与冻存2wk(85.9±3)%比较有明显下降;未冻存和冻存2wk复苏后FLS的增殖能力无明显差异。结论FLS可以进行低温冷冻,冻存时间4wk为宜。 |
| 关 键 词： | 人成纤维样滑膜细胞 冻存时间 活性 |
Influence of cryopreservation on the survival of fibroblast-like synoviocytes |
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| DAI Xing,GUO Xiao-rong,CHANG Yan,WEI Wei. Influence of cryopreservation on the survival of fibroblast-like synoviocytes[J]. Chinese Pharmacological Bulletin, 2009, 25(6) | |
| Authors: | DAI Xing GUO Xiao-rong CHANG Yan WEI Wei |
| Abstract: | Aim To determine appropriate length of cryopreservation time for fibroblast-like synoviocytes.Methods FLS were cryopreserved in-80℃ refrigerator,resuscitated and cultured after being frozed for 2,3,4 and 5 weeks.The cell viability and 24 hours adherence rate were evaluated.Results There was significant difference in the rate of cell livability of FLS cryopreserved for 5 weeks compared with that for 2 weeks.Significant difference was also observed in the 24 hours adherence rate of FLS cryopreserved for 5 weeks compared with that for 2 weeks.There was no difference in the proliferation of FLS routinely maintained compared with those cryopreserved for 2 weeks.Conclusions FLS can be cryopreserved and the time limit of cryopreservation should be 4 weeks. |
| Keywords: | fibroblast-like synoviocytes of human length of cryopreservation time viability |
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