| X射线诱导C6胶质瘤细胞凋亡的研究 |
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| 引用本文： | 黄齐兵,李新钢,李刚,王东海,王新宇,张元鹏. X射线诱导C6胶质瘤细胞凋亡的研究[J]. 中华肿瘤防治杂志, 2005, 12(7): 499-503 |
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| 作者姓名： | 黄齐兵 李新钢 李刚 王东海 王新宇 张元鹏 |
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| 作者单位： | 山东大学齐鲁医院神经外科,山东,济南,250012;山东大学齐鲁医院神经外科,山东,济南,250012;山东大学齐鲁医院神经外科,山东,济南,250012;山东大学齐鲁医院神经外科,山东,济南,250012;山东大学齐鲁医院神经外科,山东,济南,250012;山东大学齐鲁医院神经外科,山东,济南,250012 |
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| 基金项目： | 山东省卫生厅资助项目(1998CAICJA8) |
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| 摘 要： | 目的探讨X射线能否诱导胶质瘤细胞凋亡及剂量-效应关系。方法用不同剂量X射线照射细胞,并分别在不同时间段用倒置显微镜、电镜观察形态变化;DNA凝胶电泳观察阶梯状条带形成;流式细胞仪检测凋亡峰及细胞凋亡百分率。结果20Gy照射后48h观察组细胞凋亡率最高(38.49%)。结论放射可诱导胶质瘤细胞凋亡,20Gy的照射剂量对胶质瘤有最强的诱导凋亡作用,但不存在时间效应关系。
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| 关 键 词： | 神经胶质瘤 脑肿瘤 细胞死亡 |
| 文章编号： | 1009-4571(2005)07-0499-05 |
| 修稿时间： | 2004-05-26 |
Induction of apoptosis of C6 glioma cells by X radiation |
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| HUANG Qi-bing,LI Xin-gang,LI Gang,WANG Dong-hai,WANG Xin-yu,ZHANG Yuan-peng. Induction of apoptosis of C6 glioma cells by X radiation[J]. Chinese Journal of Cancer Prevention and Treatment, 2005, 12(7): 499-503 |
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| Authors: | HUANG Qi-bing LI Xin-gang LI Gang WANG Dong-hai WANG Xin-yu ZHANG Yuan-peng |
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| Affiliation: | HUANG Qi-bing,LI Xin-gang,LI Gang,WANG Dong-hai,WANG Xin-yu,ZHANG Yuan-pengDepartment of Neurosurgery,Qilu Hospital of Shandong University,Jinan 250012,P.R.China |
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| Abstract: | OBJECTIVE:To determine whether apoptosis of glioma cells can be induced by X-ray radiation,and to appropriate the dose and acting time.METHODS:The glioma cells were irradiated by different doses at different time and they were observed by the micro-scope, electromicroscope, DNA agarose gel electrophoresis and flow cytometry, and the results were analysed.RESULTS:Cell apoptosis rate of the group(20Gy,48h) was the highest,38.49%.CONCLUSION:Radiation can induce the apoptosis of C6 glioma cells and 20 Gy radio-dose has the most powerful effect on C6 glioma cells. |
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| Keywords: | glioma brain neoplasms cell death |
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