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| HPLC法测定通窍消炎丸中绿原酸的含量 | |
| 引用本文： | 刘家海. HPLC法测定通窍消炎丸中绿原酸的含量[J]. 中国中医药现代远程教育, 2013, 0(20): 157-158 |
| 作者姓名： | 刘家海 |
| 作者单位： | 河南省宁陵县食品药品检验所,宁陵476700 |
| 摘 要： | 目的 建立通窍消炎丸中绿原酸的含量测定方法.方法 采用高效液相色谱法,色谱条件为：色谱柱：Eclipse XDB-C18;流动相：乙腈-0.4%磷酸溶液（13∶87）;流速：1.0 ml·min-1;检测波长：327nm.结果 绿原酸在0.2556～0.7668mg范围内线性关系良好（r=0.9999）,平均回收率为98.75%,RSD=1.26%（n=6）.结论 本方法操作简便,专属性强,结果准确,可用于通窍消炎丸的质量控制. |
| 关 键 词： | 绿原酸 高效液相色谱法 通窍消炎丸 |
Determination of Chlorogenic Acid in Tongqiaoxiaoyan Pill by HPLC |
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| Liu Jiahai. Determination of Chlorogenic Acid in Tongqiaoxiaoyan Pill by HPLC[J]. Chinese Medicine Modern Distance Education of China, 2013, 0(20): 157-158 | |
| Authors: | Liu Jiahai |
| Affiliation: | Liu Jiahai (Ninging Institute for Food and Drug Control Ninging County,Henan Province,476700,China) |
| Abstract: | Objective To set up a method of chlorogenic acid deterrnination in Tongqiaoxiaoyan pill. Methods he content of chlorogenic acid was determined by HPLC. The chromatographic system consisted of C18 column, using acetonitrile - 0. 4% phosphatc solution（13 ： 87） as mobilc phase. The content was detected at a waveleng of 327 nm,the flow rate was 1.0ml rain 1. Results The chlorogenic acid have a good tincar rolatim in the range of 0. 2556-0. 7668mg（ r = 0. 9999）, the average recovery was 98. 75 % and RSD = 1.26 % （ n = 6）. Conolusion The established method is simple, sensitive and accuracy. It can be used for aualitv control of Tongqiaoxiaoyan pill. |
| Keywords: | Chlorogenic acid HPLC Tongqiaoxiaoyan pill |
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