柚皮素对去卵巢所致大鼠骨质疏松症的影响

目的：观察柚皮素对去卵巢所致大鼠骨质疏松症的治疗作用。方法50只雌性SD大鼠随机分为假手术组、模型组、柚皮素低、中、高剂量组,每组10只。假手术组施行假手术,其余4组均行卵巢摘除术,术后4周开始灌胃给予35、70、140 mg/kg柚皮素。12周后分别检测5组大鼠血清雌二醇(E2)、孕激素(P)、抗酒石酸酸性磷酸酶( TRACP)、碱性磷酸酶( ALP)、骨钙素( BGP),尿液钙( Ca)、磷( P)及脱氧吡啶啉( DPD)、骨密度( GMD)、股骨三点弯曲应力、L5腰椎抗压缩载荷及骨小梁形态变化。结果与假手术组比较,模型组大鼠体重明显增加,子宫脏器系数明显降低,差异有统计学意义( P <0.05)；柚皮素低、中、高剂量组大鼠体重较模型组明显降低,子宫脏器系数较模型组明显增加,差异有统计学意义( P <0.05),并呈剂量依赖性。与假手术组比较,模型组大鼠血清E2、P明显降低,差异有统计学意义( P <0.05)；柚皮素低、中、高剂量组大鼠血清E2、P较模型组明显增加,差异有统计学意义( P <0.05),呈剂量依赖性。与假手术组比较,模型组大鼠血清TRACP及尿液Ca、P、DPD明显增加,差异有统计学意义(P<0.05)；柚皮素低、中、高剂量组血清 TRACP及尿液 Ca、P、DPD较模型组明显降低,差异有统计学意义( P <0.05),且呈剂量依赖性。与假手术组比较,模型组大鼠血清ALP明显增加,BGP明显降低,差异有统计学意义( P <0.05)；柚皮素低、中、高剂量组大鼠血清ALP较模型组明显降低,BGP较模型组明显增加,差异有统计学意义( P <0.05),呈剂量依赖性。与假手术组比较,模型组大鼠BMD明显降低,差异有统计学意义( P <0.05)；柚皮素低、中、高剂量组大鼠BMD较模型组明显增加,差异有统计学意义( P <0.05),呈剂量依赖性。与假手术组比较,模型组大鼠股骨三点弯曲应力、L5腰椎抗压缩载荷明显降低,差异有统计学意义( P <0.05)；柚皮素低、中、高剂量组大鼠股骨三点弯曲应力、L5腰椎抗压缩载荷较模型组明显增加,差异有统计学意义( P <0.05),呈剂量依赖性。与假手术组比较,模型组大鼠骨小梁面积百分数、骨小梁数、骨小梁厚度明显降低,骨小梁分离度明显增加,差异有统计学意义( P <0.05)；柚皮素低、中、高剂量组大鼠骨小梁面积百分数、骨小梁数、骨小梁厚度较模型组明显增加,骨小梁分离度较模型组明显降低,差异有统计学意义( P <0.05),呈剂量依赖性。结论柚皮素对去卵巢所致大鼠骨质疏松症具有良好的治疗作用。

Effects of naringenin on osteoporosis in ovariectomized rats

Objective To evaluate the therapeutic effects of naringenin on osteoporosis in ovariectomized rats. Methods Fifty female SD rats were randomly divided into sham-operation group, model group, low-dose naringenin group, median-dose naringenin group and high-dose naringenin group, with 10 rats in each group. Except for the rats in sham-operation group, the rats in the other 4 groups underwent ovariectomy. On 4 weeks after operation, the rats in low-dose naringenin group,median-dose naringenin group and high-dose naringenin group were given naringenin by gavage, with the dosage of 35mg/kg, 70mg/kg, 140mg/kg, respectively for 12 weeks. Then the serum levels of estradiol (E2), progesterone (P), tartrate resistant acid phosphatase (TRACP), bone alkaline phosphatase (ALP), osteocalcin (BGP), urine calcium (Ca), phosphorus (P) and deoxypyridinoline (DPD), bone mineral density (BMD), the maximum bending stress,loading force of the 5th lumbar vertebra and morphologic changes of bone trabecula were detected and compared among the 5 groups. Results As compared with that in sham-operation group,the body weight in model group was obviously increased, however, uterus organ coefficient was significantly decreased ( P <0. 05). The body weight in the three medication groups was obviously decreased, however, uterus organ coefficient was significantly increased,as compared with that in model group ( P<0. 05),with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of E2 and P in model group were significantly decreased ( P <0. 05). However the serum levels of E2 and P in the three medication groups were significantly increased, as compared with those in model group ( P <0. 05),with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of TRACP,Ca,P,DPD in model group were significantly increased ( P <0. 05),however, which in the three medication groups were significantly decreased,as compared with that in model group (P<0. 05), with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of ALP in model group were obviously increased, however, the seum levels of BGP were significantly decreased ( P <0. 05). The serum levels of ALP in the three medication groups were significantly decreased,however,the serum levels of BGP were significantly increased,as compared with those in model group ( P <0. 05),with a dose-dependent manner. As compared with that in sham-operation group,the BMD was significantly decreased in model group ( P <0. 05). The levels of BMD in the three medication groups were significantly increased, as compared with those in model group ( P <0. 05), with a dose-dependent manner. As compared with those in sham-operation group,the maximum bending stress and loading force of the 5th lumbar vertebra in model group were significantly decreased ( P <0. 05). The maximum bending stress and loading force of the 5th lumbar vertebra in low-dose,median-dose and high-dose naringenin groups were significantly increased, as compared with those in model group ( P <0. 05),with a dose-dependent manner. As compared with those in sham-operation group,the percentage of bone trabecula area, bone trabecula number and thickness of bone trabecula were significantly increased in model group ( P <0. 05). The percentage of bone trabecula area, bone trabecula number and thickness of bone trabecula in the three medication groups were significantly increased, as compared with those in model group ( P <0. 05),however the resolution of bone trabecula area in the three medication groups was significantly decreased ( P <0. 05), with a dose-dependent manner. Conclusion Naringenin has better therapeutic effects on ovariectomy-induced osteoporosis in rats.