| 不同炮制方法对天麻中天麻素含量的影响 |
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| 引用本文： | 尹珉,毛克臣,陈志峰,庄志红,黄健,曹艳楠,何晓东.不同炮制方法对天麻中天麻素含量的影响[J].中国中医药信息杂志,2010,17(7):49-50. |
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| 作者姓名： | 尹珉 毛克臣 陈志峰 庄志红 黄健 曹艳楠 何晓东 |
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| 作者单位： | 1. 首都医科大学附属北京中医医院,北京,100010
2. 北京杏林药业有限责任公司,北京,101127 |
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| 摘 要： | 目的考察不同的炮制方法对天麻中主要化学成分天麻素含量的影响。方法采用RP-HPLC法。色谱柱为Agilent Eclipse XDB-C18(150mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸水溶液(3:97),流速为1.0mL/min,柱温为25℃,检测波长为230nm。对不同产地经过硫磺熏蒸与未经过硫磺熏蒸的天麻进行含量测定。结果样品平均回收率为100.29%,RSD=1.82%(n=6)。在天麻的炮制加工中,经过硫磺熏蒸的天麻,天麻素含量分别为0.15%、0.19%、0.11%,未经过硫磺熏蒸的天麻,天麻素含量分别为1.44%、0.44%、0.82%。结论在天麻的炮制加工中经过硫磺熏蒸对天麻素的含量影响较大。
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| 关 键 词： | 天麻 天麻素 炮制 硫磺 高效液相色谱法 含量测定 |
Effect of Different Processing Methods on Gastrodin Content in Gastrodia |
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| Institution: | YIN Min, MAO Ke-chen, CHEN Zhi-feng, et al(Beijing TCM Hospital Affiliated to Capital University of Medical Sciences, Beijing 100010, China) |
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| Abstract: | Objective To investigate the effect of different processing methods on the main chemical constituents of Gastrodia-gastrodin. Methods RP-HPLC method was used with columns Agilent Eclipse XDB-C18 （150 mm×4.6 mm, 5 μm）, mobile phase of acetonitrile-0.05% phosphoric acid solution （3∶97）, flow rate of 1.0 mL/min, column temperature was 25 ℃, detection wavelength was 230 nm. Gastrodia of three different areas were measured. Results The average recovery was 100.29%, RSD=1.82% （n=6）. Gastrodin contents of Gastrodia processed with sulfur fumigation were 0.15%, 0.19%, 0.11% respectively. Gastrodin contents of Gastrodia without sulfur fumigation were 1.44%, 0.44%, 0.82% respectively. Conclusion Sulfur fumigation has great impact on gastrodin content in processing of Gastrodia. |
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| Keywords: | Gastrodia Gastrodin processing sulfur HPLC content determination |
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