急性肺损伤新生大鼠肺表面活性蛋白A变化的研究

目的:探讨肺表面活性物质蛋白A(SP-A)在急性肺损伤(ALI)/ARDS发病机制中的作用及其在ALI早期诊断中的意义。方法:采用内毒素(LPS)腹腔注射的方法复制新生大鼠ALI模型,应用酶联免疫吸附法(ELISA)检测血清、BALF中的SP-A水平。结果:ALI组血清SP-A水平在1h已明显高于对照组(P<0.05),8h时达高峰值,之后虽略有下降,但仍明显高于对照组(P<0.05);ALI各组BALF中的SP-A水平与对照组相比,有下降趋势,但无统计学意义(P>0.05)。结论:ALI新生大鼠SP-A的变化,可导致PS各组分比例的改变,在ALI发病机制中发挥重要作用;ALI发病初期,血清SP-A水平即可出现显著升高,反映了肺泡上皮细胞的损伤和肺毛细血管通透性的增高。因此,检测血清SP-A水平可作为ALI早期诊断的指标。

Changes of surfactant protein a after acute lung injury in neonatal rats

Objective To explore the role of surfactant protein A(SP-A) in pathogenesis of acute lung injury(ALI) in neonatal rats.MethodAfter endotoxin lipopolysaccharide(LPS) induced acute lung injury(ALI) in neonatal rats,levels of SP-A in serum and bromchoalveolar lavage fluid (BALF) were measured by using Enzymo-linked immunoadsorbent(ELISA).ResultsIn ALI groups,level of SP-A in serum at 1 hour significantly increased than that of control group(P<0.05).The value reached peak at 8 hours.Then it decreased.Level of SP-A in BALF in all ALI groups did not significantly decrease than that of control group(P>0.05).ConclusionChanges of SP-A and PS are important for the pathogenesis of ALI.In the early of ALI,the level of SP-A in serum increases significantly.It reflects the injury of alveolar epithelial cells and the raising of the pulmonary capillary vessels′ permeability.So the concentration of SP-A in serum can be an index on the early diagnosis of ALI.