HPLC测定牛黄解毒片中黄芩苷与大黄酚的含量

目的利用梯度洗脱法,建立一种简便测定牛黄解毒片中黄芩苷和大黄酚含量的高效液相方法。方法色谱柱为ZORBAX Eclipse XDB-CBC8柱（4.6×150mm,5μm）,流动相为乙腈-磷酸缓冲溶液（磷酸0.24%,磷酸氢二钾6.31mmol·L^-1）梯度洗脱,检测波长256nm;流速1.0mL·min^-1。黄芩苷保留时间为4.417min,大黄酚保留时间为14.934min。结果黄芩苷进样量在0.020～1.000μg时呈良好的线性关系（r=0.99987）,大黄酚进样量在0.013～0.650μg时呈良好的线性关系（r=0.99982）;加样回收率分别为99.13%（黄芩苷）,99.33%（大黄酚）,RSD分别为0.59%,0.98%。结论本方法操作简便,灵敏度高,重现性好,分离效果理想。

Determination of Baicalin and Chrysophanol in Niuhuangjiedu tablets by HPLC

OBJECTIVE A quantitative HPLC method for determination of baicalin and chrysophanol in Niuhuangjiedu tablets was established. METHODS The chromatographic conditions were listed below： ZORBAX Eclipse XDB-CB C8 column, UV detector at 256nm of wavelength, a mobile phase of acetonitrile-water（0.24 % H3PO4, 6.31mmol· ^-1 K2HPO4）, using gradient elution, and 1.0mL· min^-1 of flow rate. The retention time of baicalin was 4.417min and that of chrysophanol was 14. 934min. RESULTS The calibration curves were linear for baicalin within 0.020 - 1. 000μg （ r = 0. 99987） and for chrysophanol within 0.013 - 0. 650μg （ r = 0. 99982 ） . The recoveries were 99.13 % （RSD = 0.59 % ） for baicalin and 99.33 % （RSD = 0.98 % ） for chrysophanol, respectively. CONCLUSION This method is simple, reliable, efficient, and result is satisfactory.