穿心莲内酯对TBHP诱导的椎间盘髓核细胞凋亡的作用及机制研究

目的：探讨穿心莲内酯（andrographolide,ANDRO）对叔丁基过氧化氢（tert-butyl hydroperoxide,TBHP）诱导的椎间盘髓核细胞（nucleus pulposus cells,NPC）凋亡的作用及其机制。方法：用TBHP诱导NPC建立细胞模型。将NPC分为5组：对照组、模型组（100μmol/L TBHP）、ANDRO低剂量组（100μmol/L TBHP+9μmol/L ANDRO）、ANDRO中剂量组（100μmol/L TBHP+18μmol/L ANDRO）和ANDRO高剂量组（100μmol/L TBHP+36μmol/L ANDRO）。流式细胞术检测细胞凋亡和活性氧（reactive oxygen species,ROS）水平，ELISA试剂盒检测氧化应激相关指标超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-PX）和过氧化氢酶（catalase,CAT）水平，JC-1探针法检测线粒体膜电位，Western blot检测动力相关蛋白1(dynam...

Effect of andrographolide on TBHP-induced apoptosis of intervertebral disc nucleus pulposus cells and its mechanism

A duplicate bug report detection model with enhanced text relevance semantics and multi-feature extraction was proposed to address the issues of semantic long-distance dependence and the singleness of bug report features in the current research on duplicate bug report detection. The model introduced the self-attention mechanism to capture the semantic relevance within the bug report text sequence. This mechanism calculates the contextual semantic vector dynamically for semantic analysis and resolves the problem of long-distance dependence. Additionally, the model employed the latent Dirichlet allocation algorithm to capture the topic characteristics of the bug report text. Furthermore, a feature extraction network was constructed to calculate category difference features, providing category information for the bug report simultaneously. Finally, comprehensive detection was performed based on three types of feature vectors. The experimental results demonstrate that the model achieves improved detection performance.