Galectin-3对哮喘豚鼠肺组织嗜酸粒细胞凋亡及Bax蛋白表达的影响

目的 研究Galectin-3对哮喘豚鼠肺组织嗜酸粒细胞凋亡及Bax蛋白表达的影响. 方法 将36只豚鼠随机分为0.9%氯化钠注射液组(生理盐水组)、哮喘组及Galectin-3组,每组12只.哮喘组及Galectin-3组豚鼠用卵白蛋白(OVA)致敏和激发制作哮喘模型,Galectin-3组豚鼠于致敏第15～21天激发前1 h腹腔注射Galectin-3(0.5 mg/kg),1次/d,连续7 d.测定3组豚鼠支气管肺泡灌洗液(BALF)中白介素5(IL-5)含量,计数BALF中嗜酸粒细胞数目,观察肺组织病理变化,应用缺口末端标记技术(TUNEL)法检测肺组织嗜酸粒细胞的凋亡,采用免疫组化法检测肺组织Bax蛋白的表达. 结果 Galectin-3组豚鼠BALF中IL-5含量(21.3±7.8)ng/L]显著低于哮喘组(48.6±8.9)ng/L],差异有统计学意义(P<0.01);嗜酸粒细胞计数(24.2±7.8)×10~6/L]显著低于哮喘组的(140.9±27.1)×10~6/L],差异有统计学意义(P<0.01);肺组织炎症反应明显轻于哮喘组;肺组织嗜酸粒细胞凋亡指数(14.7±4.6)%]显著高于哮喘组(3.5±0.4)%],差异有统计学意义(P<0.01);肺组织Bax蛋白表达量平均积分吸光度值(2 854.2±532.1)显著高于哮喘组(673.4±195.6),差异亦有统计学意义(P<0.01). 结论 Galectin-3能减少哮喘豚鼠BALF中嗜酸粒细胞数量,减轻肺组织炎症反应,与其降低哮喘豚鼠BALF中IL-5含量、上调肺组织Bax蛋白表达、促进肺组织嗜酸粒细胞凋亡有关.

Influence of Galectin-3 on Eosinophil Apoptosis and Expression of Bax Protein in Lung Tissues of Asthmatic Guinea Pig

Objective To study the influence of Galectin-3 on eosinophil apoptosis and Bax protein expression in lung tissue of asthmatic guinea pig.Methods Thirty-six guinea pigs were randomly divided into groups saline (receiving 0.9% sodium chloride injection),asthma,and Galectin-3,12 in each.Asthma models were established by sensitization and stimulation with ovalbumin (OVA) in groups asthma and Galectin-3.Galectin-3 group were given intraperitoneal injection of Galectin-3 (0.5 mg/kg,once/d,continuously for 7 d) be...