蒲公英提取物对MKN-45胃癌细胞株P53和Ki67表达的影响

目的检测蒲公英提取物对MKN45胃癌细胞的P53、Ki67两种蛋白的表达的影响,探求中草药蒲公英抗癌作用的依据。方法以小鼠血清药物学方法配制蒲公英提取物药液,对MKN45胃癌细胞进行培养传代,取处于对数生长期的同一代细胞加入药液血清,应用免疫组化(S-P法)检测p53、Ki67的表达状况。结果 4组中p53的表达分别为100%(5/5)、60%(3/5)、80%(4/5)和60%(3/5),所有含药组合并后其P53阳性表达率与对照组之间具有显著性差异(p<0.05),各含药组的Ki67阳性表达率均高于对照组,分别为87%、96%、97%、92%,但目前尚未发现它们之间具有显著性差异(p>0.05)。结论蒲公英可能有降低胃癌细胞p53的阳性表达的作用。

EFFECT OF DANDELION EXTRACT ON EXPRESSION OF P53 AND KI67 IN GASTRIC CANCER MKN45 CELL LINES

Objective The effect of dandelion extract （DE） on expression of P53 and Ki67 in gastric cancer MKN45 cell were studied as a way to evaluate the antitumor effects of Mongolian dandelion herb. Methods 20 mice were used to prepare four groups of the mouse blood serum（ including a normal control group and three groups of different concentrations of DE）. After the ser- ums were added in the gastric cancer MKN45 cell culture media respectively, the expression of P53 protein and Ki67 protein were ex- amined respectively by streptavidin - peroxidase conjugated method （SP） . Results The expression of p53 was found 100% （ 5/5 ）, 60% （3/5）, 80% （4/5） and 60% （3/5） respectively in the four groups. The positive rate of p53 in DE groups（all the groups to- gether） was lower than that in control group; the expression of Ki67 was found 87.0%, 96. 0% , 97.5% and 92. 0% in the four groups. The expression of Ki67 in each group of the three DE groups was higher than that in control group. Conclusion There is a weak experimental evident to show that TE decrease the expression of P53 in gastric cancer MKN45 cell while increased the expression of Ki67.