冬凌草甲素对人胰腺癌SW1990细胞系生长抑制作用

目的研究冬凌草甲素(oridonin)对人胰腺癌SW1990细胞生长抑制作用并探究其可能机制。方法采用MTT法检测冬凌草甲素对SW1990细胞增殖抑制作用、Hoechst 33258染色法及透射电镜观察细胞的形态学变化、流式细胞仪检测细胞周期及凋亡率、RT-PCR法检测survivin、p21mR-NA表达。结果冬凌草甲素对人胰腺癌SW1990细胞具有明显的生长抑制作用,荧光显微镜和透射电镜下均见到典型的凋亡形态学改变,流式分析出现亚G1峰并显示G2/M期周期阻滞。RT-PCR分析结果显示p21mRNA表达增加而survivin mRNA表达减少。结论冬凌草甲素通过诱导凋亡和G2/M期周期阻滞来抑制人胰腺癌SW1990细胞生长,其分子机制可能与survivin和p21调节的信号途径有关。

The inhibitory effect of oridonin on human pancreas adenocarcinoma SW1990 cells

Aim To study the inhibitory effects and its mechanisms of oridonin on human pancreas adenocarcinoma SW1990 cells.Methods Cell growth inhibition mediated by oridonin on SW1990cells was measured by MTT assay.The morphological changes were observed by Hoechst33258 fluorochrome staining and electron microscope.Cell cycle and apoptosis rate were analyzed by flow cytometry. The molecular mechanisms involved in the effects of oridonin on SW1990 cells were studied by RT-PCR.Results The growth of humen pancreas adenocarcinoma SW1990 cells was significantly inhibited by oridonin.Apoptosis morphological changes about chromatic agglutination and nuclear condensation were detected by Hoechst 33258 fluorochrome staining and electron microscope in oridonin treated SW1990 cells."Sub-G_1" phase peak and G_2/M growth arrest werer found with flow cytometry.The upregulating mRNA expression of p21 and downregulating mRNA expression of survivin were detected by RT-PCR.Conclusion The inhibitory effect of oridonin on human pancreas adenocarcinoma SW1990 cells through induced apoptosis and G_2/M growth arrest and the mechanisms may be through surviving-p21 co-regluation pathway.