单纯疱疹病毒性角膜炎的实验室检查现状及进展

单纯疱疹病毒性角膜(herpes simplex keratitis,HSK)是一种严重的感染性致盲性眼病。过去HSK的诊断主要依据临床特征,然而这并不可靠。随着分子生物学的发展,目前对于单纯疱疹病毒性角膜炎的诊断有了突飞猛进的发展。共焦显微技术、环媒恒温扩增技术、实时荧光探针定量聚合酶链式反应(polymerase chain reaction,PCR)、巢式PCR(nested PCR)及多重聚合酶链式反应(multiplex PCR)等被认为是较敏感且快速的诊断方法。

Progress on the diagnosis of herpes simplex keratitis

Herpes simplex keratitis(HSK)is the leading cause of infectious blindness. In general, diagnosis of HSK is based on clinical findings, which often are not confirmatory. With the development of molecular biology, a variety of laboratory methods that helped in achieving a diagnosis including in vivo confocal microscopy(IVCM), loop-mediated isothermal amplification(LAMP), real-time polymerase chain reaction(rt-PCR), nested PCR and multiplex PCR. They are considered as accurate and fast diagnostic techniques, thus characteristics of these are reviewed in this paper.