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Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination |
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| Authors: | Daisuke Suzuki Toshi Nagata Ghada Eweda Sohkichi Matsumoto Makoto Matsumoto Kunio Tsujimura Yukio Koide |
| Affiliation: | 1. 3rd Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu, Japan;2. Department of Health Science, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu, Japan;3. Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu, Japan;4. Department of Host Defense, Osaka City University Graduate School of Medicine, Osaka, Japan;5. Otsuka Pharmaceutical Co., Ltd., Kagasuno 463-10 Kawauchi-cho, Tokushima, Japan |
| Abstract: | Mycobacterial DNA-binding protein 1 (MDP1) is a major protein antigen in mycobacteria and induces protective immunity against Mycobacterium tuberculosis infection in mice. In this study we determined murine T-cell epitopes on MDP1 with MDP1 DNA immunization in mice. We analyzed interferon-γ production from the MDP1 DNA-immune splenocytes in response to 20-mer overlapping peptides covering MDP1 protein. We identified several CD4+ T-cell epitopes in three inbred mouse strains and one CD8+ T-cell epitope in C57BL/6 mice. These T-cell epitopes would be feasible for analysis of the role of MDP1-specific T-cells in protective immunity and for future vaccine design against M. tuberculosis infection. |
| Keywords: | DNA immunization T-cell epitope Mycobacterium tuberculosis |
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