黄芪、脉络宁注射液载入修饰胶原对鸡胚尿囊膜血管新生的协同促进作用

背景：课题组先期研究发现黄芪载入修饰后胶原可以促进血管新生，与生长因子载入疗效相当，运用中医理论中具有协同作用的中药合用是否能进一步提高疗效尚不清楚。 目的：探查黄芪、脉络宁载入修饰后胶原促鸡胚尿囊膜血管新生及长入胶原的疗效，并证明黄芪与脉络宁是否有协同作用。 方法：实验分空白组、控制组(单纯胶原)、黄芪组(黄芪注射液1 mL载入胶原)、脉络宁组(脉络宁注射液1 mL载入胶原)、黄芪+脉络宁组(黄芪注射液及脉络宁注射液各0.5 mL载入胶原)，各组植入鸡胚尿囊膜孵化7 d后取出，测定鸡胚尿囊膜内微血管数、各组胶原内微血管数、血红蛋白含量、rhVEGF165阳性细胞数。 结果与结论：各实验组鸡胚尿囊膜内血管呈轮辐状生长及鸡胚尿囊膜包裹标本率高于空白组与控制组，鸡胚尿囊膜内微血管数、胶原内微血管数、血红蛋白含量、rhVEGF165阳性细胞数均高于控制组，差异有显著性意义(P < 0.01)；其中黄芪+脉络宁组又高于黄芪组与脉络宁组，差异有显著性意义(P < 0.05)。提示黄芪、脉络宁载入胶原后可以促进鸡胚尿囊膜内血管新生并刺激血管长入胶原内，黄芪与脉络宁合用具有协同作用，机制之一为刺激血管内皮细胞血管内皮生长因子的表达。

Synergistic role of Huangqi and/or Mailuoning injection with modified collagen in enhancing angiogensis of chicken embryo allantois membrane

BACKGROUND: Previous study has found that Huangqi loading modified collagen can promote angiogenesis, and the effect is equal to growth factor-loading effect. It remains unclear that using synergistic Chinese medicine in accordance with traditional Chinese medicine theory is able to further improve the efficacy. OBJECTIVE: To explore the angiogenic effects of the micro-vessels in the chick chorioallantoic membrane and collagen matrices by loading the Chinese herbal injection of Huangqi and Mailuoning into modified collagen. METHODS: Five groups were set in the experiment, including blank group without any interfere, control group with only modified collagen implanted into chorioallantoic membrane, Huangqi group with 1 mL of Huangqi injection loading in collagen, Mailuoning group with 1 mL of Mailuoning injection loading in collagen, Huangqi plus Mailuoning group with 0.5 mL Huangqi and 0.5 mL Mailuoning injection loading in collagen. After implanted for 7 days, the samples were explanted from the chorioallantoic membrane. The number of micro-vessels in chorioallantoic membrane was counted, the hemoglobin content and the number of recombinant human vascular endothelial growth factor (rhVEGF165) positive cells in collagen were detected. RESULTS AND CONCLUSION: For the appearance of macro-samples, the rate of wheel spoke-like vessels and enclosing area of samples by chorioallantoic membrane in each experimental group were better than blank group and control group. The number of micro-vessels in the chorioallantoic membrane, together with hemoglobin content and the number of rhVEGF165 positive cells in the collagen matrices were significantly higher in each experimental group than those in the control group (P < 0.01). Among the experimental groups, the index of Huangqi plus Mailuoning group was significantly higher than the results of Huangqi alone and Mailuoning alone groups (P < 0.05). Loading Huangqi and/or Mailuoning injection in modified collagen can improve angiogenic effects in the chorioallantoic membrane, with the ability of stimulating vessels to grow into the modified collagen. Huangqi plus Mailuoning have a synergetic effect. One of the possible mechanisms is to enhance the expression of rhVEGF165 of vascular endothelial cells.