缺血后适应对大鼠肾脏急性热缺血再灌注损伤细胞凋亡的影响

目的 探讨缺血后适应对大鼠急性肾缺血再灌注损伤(isclaemic reperfusion injury,IRI)所导致的细胞凋亡的影响.方法 将40只Wistar健康雄性大鼠随机分为对照组(C组)、缺血再灌注组(IRI组)、缺血后适应组(IPo组)和腺苷组(Ado组)4组,C组仅行右肾切除,左肾蒂游离,其他3组除切除右肾外,IRI组为大鼠肾脏急性热缺血再灌注损伤模型,IPo组于再灌注之前行6个10 s无限制灌注 10 s再缺血循环,Ado组于缺血前10 min经静脉给予腺苷.分别采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)、流式细胞术及肾组织病理形态学的方法观察细胞凋亡.结果 ,与C组相比,IRI组细胞凋亡数显著增加(P＜0.05);与IRI组相比,IPo组和Ado组细胞凋亡数显著减少(P<0.05),而二者之间差异无统计学意义,结论 缺血后适应可以减少缺血再灌注引起的大鼠肾脏细胞凋亡.再灌注损伤;细胞凋亡

The Effect of Ischemic Postconditioning on Apoptosis Induced by Acute Hot Renal Ischemic-reperfusion

Objective To investigate the effects of ischemic postconditioning(IPo) on cellular apoptosis in acute renal ischemic-reperfusion injury(IRI) in rats.Methods Model of acute renal ischemic-reperfusion injury(IRI) was constructed using Wistar rats.Forty healthy male rats were divided into 4 groups randomly,including group C,group IRI,group IPo and group Ado.Rats of group C received right nephronectomy and separation of left renal vessels.Before reperfusion,group IPo was perfused for 10 s,following ischemic reperfusion for another 10 s with 6 cycles.Group Ado was infused with adenosine intravenously 10 min before ischemia.Cellular apoptosis was determined with TUNEL(TdT-mediated dUTP Nick End Labelling),flowcytometry and histopathological staining.Results Compared with group C,the apoptotic index increased predominatly in group IRI(P<0.05),whereas,apoptosis index decreased obviousely in group IPo,Ado(P<0.05).In addition,the apoptosis index between group IPo and Ado was not statistically different.Conclusion Ischemic postconditioning could decrease cellular apoptosis induced by IRI in rat kidney.