化学沉淀法测定小而密低密度脂蛋白方法的建立

目的利用化学沉淀原理建立小而密低密度脂蛋白(sdLDL)快速、简便的检测方法。方法通过比较不同种类及不同浓度沉淀剂对血清样本中脂蛋白的分离效果,确定最终沉淀剂,并对化学沉淀法测定血清sdLDL-C的精密度、线性范围和回收率进行分析。结果最终选择140 U/mL肝素-90 mmol/L Mg2+作为沉淀剂测定血清sdLDL胆固醇(sdLDL-C)含量。化学沉淀法测定sdLDL-C在0.14~1.44 mmol/L范围内线性关系良好,回归方程为Y=1.025X-0.197,r=0.988;对高、低2组不同sdLDL-C浓度的样本批内变异系数(CV)分别为2.83%、3.19%,批间CV分别为5.49%、6.13%;回收率为83.81%。结论 sdLDL化学沉淀法具有样本用量少、操作简便、成本低廉等优点,更适合常规实验室检测血清sdLDL-C含量。

Establishment on chemistry precipitation assay for the determination of small dense low-density lipoprotein

Objective To establish a simple and rapid method for the determination of small dense low-density lipoprotein（sdLDL）by utilizing the principle of precipitation.Methods The final and most appropriate precipitation reagents were identified through comparing the separation effect of different types and different concentrations of precipitation reagents for separating serum lipoproteins.The precision,linear range and recovery rate of chemistry precipitation assay were also analyzed.Results Finally,1 40 U/mL of heparin and 90 mmol/L of Mg2＋ as the precipitation reagents were used to determine the concentrations of serum sdLDL cholesterol（sdLDL-C）.The chemistry precipitation assay was linear at 0.1 4-1 .44 mmol/L sdLDL-C,and the regression equation was Y=1 .025X-0.1 97,r=0.988.The within-run coefficients of variation（CV）of high and low sdLDL-C concentration specimens were 2.83% and 3.1 9%,respectively,and the between-run CV were 5.49%and 6.1 3%.The recovery rate was 83.81%.Conclusions The chemistry precipitation assay for the determination of sdLDL has the advantages of less serum consumption,simple operation,low cost and so on.It is more suitable for clinical laboratory to determine serum sdLDL-C.