慢性乙型肝炎患者外周血T淋巴细胞亚群变化与病毒复制的关系

目的探讨慢性乙型肝炎患者外周血T淋巴细胞亚群变化与乙型肝炎病毒（HBV）复制的关系。方法用流式细胞术对81例慢性乙型肝炎患者和30名健康对照者进行外周血T淋巴细胞亚群检测,用荧光定量聚合酶链反应（PCR）测定血清中的HBV DNA含量。结果慢性乙型肝炎患者外周血CD3＋、CD4＋T细胞百分率及CD4＋/CD8＋比值较健康对照者显著降低,而CD8＋T细胞百分率显著升高。与健康对照组相比,HBV DNA阴性患者只有CD4＋/CD8＋比值变化显著;HBV DNA阳性患者CD3＋、CD4＋、CD8＋T细胞百分率及CD4＋/CD8＋比值有显著改变,但高拷贝组和低拷贝组相比,差异无统计学意义。结论慢性乙型肝炎患者感染HBV后免疫功能下降与病毒复制有关,HBV复制可进一步加重免疫功能紊乱。

Relationship between HBV DNA replication and T lymphocyte subsets in peripheral blood of patients with chronic hepatitis B

Objective To explore the relationship between replication of Hepatitis B virus （ HBV ） DNA and T lymphocyte subsets in peripheral blood of patients with chronic hepatitis B. Methods The relative percentage of T lymphocyte subsets in peripheral blood of 81 patients with chronic hepatitis B and 30 healthy controls was analyzed by flow cytometry. The serum load of HBV DNA was measured by fluorescence quantitative polymerase chain reaction （PCR）. Results Compared with healthy controls, the patients with chronic hepatitis B had a decreased percentage of CD3^＋ and CD4^＋ T cells, a decreased CD4^＋/CD8^＋ ratio, and an increased percentage of CD8^＋ T cells. Compared with healthy controls, the HBV DNA-negative patients with chronic hepatitis B had a significant change only in CD4^＋/CD8^＋ ratio. The HBV DNA-positive patients with chronic hepatitis B had a significant variation in the percentages of CD3 ^＋ , CD4^＋ , CD8^＋ T cells and CD4^＋/CD8^＋ ratio, but there were no significant differences in the percentages of CD3^＋, CD4^＋, CD8^＋ T cells and CD4^＋/CD8^＋ ratio between high copies group and low copies group. Conclusions Down-regulation of immunological function of patients with chronic hepatitis B is correlated to the replication of HBV. The disorder of immunological function can be promoted by HBV DNA replication.