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| CUEDC2通过活化 PI3K／Akt 信号通路促进乳腺癌细胞的生长 | |
| 引用本文： | 李彦姝,高云玲,张红艳,汤丽娜,刘芙蓉,李丰. CUEDC2通过活化 PI3K／Akt 信号通路促进乳腺癌细胞的生长[J]. 现代肿瘤医学, 2017, 0(2): 183-185. DOI: 10.3969/j.issn.1672-4992.2017.02.005 |
| 作者姓名： | 李彦姝 高云玲 张红艳 汤丽娜 刘芙蓉 李丰 |
| 作者单位： | 中国医科大学基础医学院细胞生物学教研室，教育部医学细胞生物学重点实验室，辽宁 沈阳 110122 |
| 基金项目： | 国家自然科学基金资助项目(编号31371424) |
| 摘 要： | 目的：探讨 CUE 结构域2（CUE domain －containing 2，CUEDC2）蛋白在人乳腺癌细胞中的生物学作用。方法：人乳腺肿瘤细胞 MCF －7转染的 Myc －CUEDC2真核表达载体，提取转染细胞的总蛋白检测磷酸化 Akt 和总 Akt 的表达变化。利用 CCK －8检测过表达 CUEDC2后 MCF －7细胞的生长。结果：CUEDC2能够活化 Akt，使磷酸化 Akt 表达增加，能够促进人乳腺癌细胞生长。结论：当乳腺癌中 CUEDC2表达增高时，能够引起 PI3K／Akt 信号通路活化，促进肿瘤细胞生长。 |
| 关 键 词： | 乳腺肿瘤 CUEDC2 细胞生长 |
CUEDC2 promotes breast cancer progression through activation of PI3K/Akt signaling pathway |
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| Li Yanshu,Gao Yunling,Zhang Hongyan,Tang Li'na,Liu Furong,Li Feng. CUEDC2 promotes breast cancer progression through activation of PI3K/Akt signaling pathway[J]. Journal of Modern Oncology, 2017, 0(2): 183-185. DOI: 10.3969/j.issn.1672-4992.2017.02.005 | |
| Authors: | Li Yanshu Gao Yunling Zhang Hongyan Tang Li'na Liu Furong Li Feng |
| Abstract: | Objective:To evaluate the biological role of CUEDC2 in human breast cancer cells.Methods:Human breast cancer cell line MCF -7 were transient transfected with Myc -CUEDC2 constructs,and whole -cell lysates were extracted with the level of phosphorylated Akt (p -Akt)and the total Akt proteins.CCK -8 assay was used for detecting the role of CUEDC2 in breast cancer cell line progression.Results:CUEDC2 induced an increase in the lev-el of phosphorylated Akt (p -Akt)and increased the growth of breast cancer cells.Conclusion:Our results suggest that increased expression of CUEDC2 contributes to tumor growth by activation of the PI3K/Akt pathway in breast cancer cell lines,which may be one of the mechanisms that attribute to promote the growth of cancer cells. |
| Keywords: | breast cancer CUE domain -containing 2 cell growth |
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