CUEDC2通过活化 PI3K／Akt 信号通路促进乳腺癌细胞的生长

目的：探讨 CUE 结构域2（CUE domain －containing 2，CUEDC2）蛋白在人乳腺癌细胞中的生物学作用。方法：人乳腺肿瘤细胞 MCF －7转染的 Myc －CUEDC2真核表达载体，提取转染细胞的总蛋白检测磷酸化 Akt 和总 Akt 的表达变化。利用 CCK －8检测过表达 CUEDC2后 MCF －7细胞的生长。结果：CUEDC2能够活化 Akt，使磷酸化 Akt 表达增加，能够促进人乳腺癌细胞生长。结论：当乳腺癌中 CUEDC2表达增高时，能够引起 PI3K／Akt 信号通路活化，促进肿瘤细胞生长。

CUEDC2 promotes breast cancer progression through activation of PI3K/Akt signaling pathway

Objective:To evaluate the biological role of CUEDC2 in human breast cancer cells.Methods:Human breast cancer cell line MCF -7 were transient transfected with Myc -CUEDC2 constructs,and whole -cell lysates were extracted with the level of phosphorylated Akt (p -Akt)and the total Akt proteins.CCK -8 assay was used for detecting the role of CUEDC2 in breast cancer cell line progression.Results:CUEDC2 induced an increase in the lev-el of phosphorylated Akt (p -Akt)and increased the growth of breast cancer cells.Conclusion:Our results suggest that increased expression of CUEDC2 contributes to tumor growth by activation of the PI3K/Akt pathway in breast cancer cell lines,which may be one of the mechanisms that attribute to promote the growth of cancer cells.