FGFR1和MMP3在食管鳞状细胞癌中的表达及临床意义

目的:观察成纤维细胞成长因子受体1(fibroblast growth factor receptor1,FGFR1)和基质金属蛋白酶3(matrix metalloproteinase 3,MMP3)基因和蛋白在食管鳞癌、癌旁及正常食管组织中的表达情况,探讨它们与食管鳞癌患者临床病理特征之间的关系.方法:采用RT-qPCR、Western blot和免疫组织化学染色检测78例食管鳞癌组织(鳞癌组)、35例食管鳞癌癌旁组织(癌旁组)及35例正常食管组织(正常组)中FGFR1和MMP3 mRNA及蛋白的表达,并分析其与肿瘤临床病理特征之间的关系.结果:FGFR1和MMP3 mRNA在食管鳞癌组表达水平显著高于癌旁组和正常组,差异具有统计学意义(P＜0.05).在癌旁组和正常组表达差异无统计学意义(P＞0.05).FGFR1和MMP3蛋白在食管鳞癌组中的表达率和表达水平明显高于癌旁组和正常组,差异具有统计学意义(P＜0.05).在癌旁组及正常组表达率及表达水平差异均无统计学意义(P＞0.05).FGFR1和MMP3蛋白在食管鳞癌中的表达与患者性别、年龄、肿瘤分化程度无关(P＞0.05),而与肿瘤浸润深度、淋巴结是否转移及TNM分期相关(P＜0.05).食管鳞癌中,FGFR1和MMP3的蛋白表达呈正相关(r=0.303,P＜0.05).结论:FGFR1和MMP3在食管鳞癌中均高表达,两者可能与食管癌的侵袭和转移有关.

Expression and clinical significance of FGFR1 and MMP3 in esophageal squamous cell carcinoma

Objective:To investigate the expression of fibroblast growth factor receptor 1 (FGFR1) and matrix metalloproteinase 3 (MMP3) in esophageal squamous cell carcinoma tissues,para-tumor tissues and normal esophageal tissues,and the association with clinical pathological features.Methods:Real-time qPCR,immunohistochemistry and Western blot were used to detect the expressions of FGFR1 and MMP3 mRNA and protein levels in esophageal squamous cell carcinoma tissues (78 cases),the corresponding para-tumor tissues (35 cases) and normal esophageal tissues (35 cases).Their relationships with clinical pathological characteristics of esophageal squamous cell carcinoma were analyzed.Results:The expression of FGFR1 and MMP3 mRNA in esophageal squamous cell carcinoma tissues were much higher than those in para-tumor tissues and normol esophageal tissues (P ＜ 0.05).No significant difference was observed between para-tumor tissues and normol esophageal tissues (P ＞ 0.05).The positive rate and expression level of FGFR1 and MMP3 protein in esophageal squamous cell carcinoma tissues were significantly higher than those in para-tumor tissues and normal esophageal tissues (P ＜ 0.05).No significant difference was observed between para-tumor tissues and normol esophageal tissues (P ＞ 0.05).The expression of FGFR1 and MMP3 protein in esophageal squamous cell carcinoma was not associated with gender,age,differentiation of carcinoma (P ＞0.05),but associated with the depth of invasion,lymphatic metastasis and tumor TNM stages (P ＜ 0.05).The expression level of FGFR1 in esophageal squamous cell carcinoma was positively correlated with the expression of MMP3 (r =0.303,P ＜ 0.05).Conclusion:The expression of FGFR1 and MMP3 are significantly higher than those in para -tumor tissues and normal esophageal tissues.They may be associated with invasion and metastasis of esophageal squamous carcinoma.