miR－647在结肠癌细胞中的表达及临床意义

目的：分析miR－647在结肠癌组织及细胞系中的表达情况，探讨其对结肠癌细胞增殖、迁移能力的影响及其可能的作用机理。方法：利用Real－time quantitative Polymerase Chain Reaction（qPCR）技术检测17例结肠癌患者癌及癌旁组织中miR－647的表达；利用qPCR技术检测正常人肠上皮细胞HIEC及结肠癌细胞HT－29中miR－647的表达；将miR－647 antagomir及对照分别转染至结肠癌细胞中，应用MTT实验检测细胞增殖，体外划痕实验检测细胞迁移能力，评价转染miR－647抑制剂对结肠癌细胞增殖能力和迁移能力的影响。结果：qPCR结果显示，与癌旁组织相比，miR－647在结肠癌肿瘤组织中表达明显升高；与正常人肠上皮细胞相比，人结肠癌细胞系HT－29中miR－647表达明显升高；MTT结果显示miR－647抑制剂可以显著抑制SW480和SW620细胞的增殖能力和细胞迁移能力。结论：miR－647通过促进结肠癌细胞的增殖和迁移能力，参与结肠癌的发生发展进程。

The expression and clinical significance of miR-647 in colon cancer cells

Objective:To analyze the expression of miR-647 in colon cancer tissues and colon cancer cell lines, explore the influences of miR-647 on cell proliferation and migration,and discuss the possible mechanism.Meth-ods:Detecting the expression of miR-647 in 17 pairs of colon cancer tissues and adjacent tissues by Real -time quantitative Polymerase Chain Reaction (qPCR).Analyzing the expression of miR-647 in HIEC and HT-29 by qPCR.Transfecting miR-647 antagomir and negative control into colon cancer cells by using the LipofectaminTM RNAiMAX,and then testing the cell proliferation and migration by MTT and wound healing assay respectively.Re-sults:qPCR showed that miR-647 was significantly increased in tumor tissue of patients with colon cancer compared with the adjacent tissues.And miR-647 was also notably increased in colon cancer cell line (HT-29 )compared with the HIEC.MTT and wound healing assay results showed that miR-647 antagomir significantly inhibited the pro-liferation and migration of SW480 and SW620 cells.Conclusion:miR-647 participated in the process of colon canc-er development by promoting the proliferation and migration abilities of colon cancer cell lines.