芹菜素通过 PI3K/Akt 信号通路诱导 Hela 细胞凋亡

目的：初探芹菜素（Apigenin）对人宫颈癌细胞 Hela 的抑制机制。方法：MTT 法检测 Apigenin 对 Hela细胞的增殖抑制作用，实时无标记细胞分析技术测量 Apigenin 对 Hela 细胞生长曲线的影响。Western blot 检测 Apigenin 对 Hela 细胞中 PI3K/ Akt 信号通路的影响。结果：与阴性对照组相比，Apigenin 能显著抑制 Hela细胞的增殖（P <0.01），当药物浓度为20μmol/ L 时抑制率达到（80.5±5.3）%，IC50值为（6.8±0.8）μmol/ L。细胞生长曲线反映，Apigenin 减缓了 Hela 细胞的增殖，对数期细胞经20μmol/ L Apigenin 处理后，细胞数量迅速下降。Western blot 结果显示，Apigenin 能显著抵抗 IGF -1引起的 Akt 激活但并不能降低 PI3K 的磷酸化水平。同时 Apigenin 还能降低 Bcl -2/ Bax 比例，提高 Caspase -3活性，启动细胞线粒体凋亡。结论：Apigenin可以通过 PI3K/ Akt 信号通路促进 Hela 细胞的凋亡。

Apigenin induces Hela cells apoptosis via PI3K/Akt signaling pathway

Objective:To observe the inhibitory effect of Apigenin on the Hela cell lines,and investigate the poten-tial mechanism. Methods:MTT assay was used to detect the anti - proliferative effect of Apigenin on Hela cells. The influence of Apigenin on growth curve of Hela cells was detected by real time cellular analysis. The PI3K/ Akt signal pathway was analyzed by Western blot assay. Results:Compare with negative control,apigenin showed a significantly inhibitory ability to Hela cells(P < 0. 01). After treated with Apigenin for 48 h,the IC50 value to Hela cells were (6. 8 ± 0. 8)μmol/ L,inhibition rate rised to(80. 5 ± 5. 3)% while the concentration is 20μmol/ L. Cell growth curve indicate that Apigenin is able to slow down the proliferation of Hela,after treated with 20μmol/ L Apigenin,the cell count were quickly reduced. Apigenin could intensively resist Akt activation which caused by IGF - 1,but had no effect on PI3K,Apigenin could also launch the mitochondrial apoptotic by reducing the Bcl - 2 / Bax proportion and in-creasing the activity of caspase family. Conclusion:Apigenin could inhibit the proliferation of Hela cells and induce apoptosis,the potential mechanism is probably related with regulation of PI3K/ Akt pathway.apoptosis,the potential mechanism is probably related with regulation of PI3K/ Akt pathway.