| 高效液相色谱法测定熊去氧胆酸片的含量 |
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| 引用本文： | 张燕,佟爱东. 高效液相色谱法测定熊去氧胆酸片的含量[J]. 中国药品标准, 2012, 13(5): 359-362 |
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| 作者姓名： | 张燕 佟爱东 |
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| 作者单位： | 广州市药品检验所,广州510160 |
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| 摘 要： | 目的:建立测定熊去氧胆酸片含量的方法。方法:色谱柱为Hypersil ODS2(4.6 mm×250 mm,5μm),流动相为0.001mol.L-1磷酸二氢钾溶液(磷酸调pH至2.0)–乙腈(50∶50),检测波长为210 nm,柱温40℃。结果:熊去氧胆酸进样量在1.8μg～104μg范围内线性良好(r=0.9998),平均回收率(n=9)为102.0%(RSD=1.2%)。结论:该方法准确、灵敏,重现性好,可有效控制熊去氧胆酸片的质量。
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| 关 键 词： | 熊去氧胆酸片 含量测定 高效液相色谱法 |
HPLC Determination of Content of Ursodeoxycholic Acid Tablets |
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| Zhang Yan and Tong Aidong. HPLC Determination of Content of Ursodeoxycholic Acid Tablets[J]. Drug Standards of China, 2012, 13(5): 359-362 |
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| Authors: | Zhang Yan and Tong Aidong |
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| Affiliation: | ( Guangzhou Institute for Drug Control, Guangzhou 510160) |
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| Abstract: | Objective： To establish an HPLC method for determination of the content of Ursodeoxycholic Acid Tablets. Method： A Hypersil ODS2 column （4. 6 mm x 250 ram,5μm）was used. Using the mobile phase of 0. 001mol L-1 potassium dihydrogen phosphate solution （adjust to pH 2.0 with phosphate acid acetonitrile （50： 50）. The detection wavelength was set at 210nm and temperature was 40 ℃. Result： Ursodeoxyeholie acid showed a good linear relationship in the range of 1.8-104μg（ r = 0. 999 8 ） , and the average recovery wasl02. 0% with RSD = 1.2% （n =9）. Conclusion： The method is specific, accurate and convenient to be used for the quality control of Ursodeoxycholic Acid Tablets. |
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| Keywords: | Ursodeoxycholic Acid Tablets content determination HPLC |
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