| 淫羊藿总黄酮对大鼠成骨细胞代谢调控的机制研究 |
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| 引用本文： | 刘亦恒,张海英,臧洪敏,陈君长. 淫羊藿总黄酮对大鼠成骨细胞代谢调控的机制研究[J]. 中国中药杂志, 2006, 31(6): 487-490 |
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| 作者姓名： | 刘亦恒 张海英 臧洪敏 陈君长 |
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| 作者单位： | 1. 西安交通大学,第二医院,陕西,西安,710004
2. 西安交通大学,医学院,陕西,西安,710061 |
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| 摘 要： | 目的：探讨淫羊藿总黄酮对大鼠成骨细胞代谢调控的影响。方法:取新生SD大鼠的颅骨,胶原酶法培养成骨细胞。应用MTT法、对硝基苯磷酸盐法(PNPP)和RT-PCR方法观察淫羊藿总黄酮对体外培养成骨细胞的增殖、碱性磷酸酶活性和OPG mRNA,RANKL mRNA表达的影响。结果:淫羊藿总黄酮能刺激成骨细胞增殖,提高碱性磷酸酶活性,增强OPG mRNA的表达（P<0.05或P<0.01）。结论:淫羊藿总黄酮能促进成骨细胞的增殖、分化,增加OPG mRNA的表达,对RANKL的表达无明显影响。
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| 关 键 词： | 淫羊藿总黄酮 成骨细胞 OPG RANKL 细胞培养 |
| 文章编号： | 1001-5302（2006）06-0487-04 |
| 收稿时间： | 2005-07-01 |
| 修稿时间： | 2005-07-01 |
Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro |
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| LIU Yi-heng ; ZHANG Hai-ying ; ZANG Hong-min ;CHENG Jun-chang. Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro[J]. China Journal of Chinese Materia Medica, 2006, 31(6): 487-490 |
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| Authors: | LIU Yi-heng ZHANG Hai-ying ZANG Hong-min CHENG Jun-chang |
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| Affiliation: | Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. liuyiheng-xjtu@sohu.com |
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| Abstract: | Objective: To explore the effect of Herba Epimedii flavone(HEF) on the osteoblast metabolism in vitro.Method: Osteoblast were obtained from new born rat calvaria by digestive enzymes.MTT,PNPP and RT-PCR were used to observe the proliferation,activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.Result: It was found that HEF had the effect on stimulating cell proliferation,activity of ALP and the mRNA expression of OPG of cultured osteoblasts(P<0.01,P<0.05).Conclusion: HEF can promote the proliferation,the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro. |
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| Keywords: | Herba Epimedii flavone osteoblast osteoporogeterin recertor activator nuclear factor kappa B ligand cell culture |
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