黄芩茎叶总黄酮对铝中毒小鼠记忆障碍的作用

目的研究黄芩茎叶总黄酮(TotalflavonoidsfromstemsandleavesofScutellariabaicalensisGeorgi,SSF)对慢性铝中毒小鼠学习记忆运动障碍、神经和肝脏病理改变及自由基不正常变化的作用。方法小鼠腹腔注射(Introperitonealinjection,ip)AlCl350d制备铝中毒模型。通过小鼠学习记忆能力、自主活动、皮层和肝脏病理改变及脑肝脏丙二醛(malondialdehyde,MDA);超氧化物岐化酶(superoxidedismutase,SOD)测定,评价SSF对小鼠慢性铝中毒的作用。结果与空白对照组相比,AlCl3(100mg·kg-1,ip,50d)使小鼠学习记忆能力降低、自主活动次数减少、皮层和肝脏细胞病理改变、脑和肝脏MDA水平增加和SOD活性减低。SSF50、100和200mg·kg-1不同程度的改善铝中毒小鼠上述病理改变。结论SSF能够改善慢性铝中毒小鼠学习记忆运动障碍、神经肝脏病理改变和自由基不正常变化。

Effects of SSF on memory deficits in aluminum toxic mice

Aim To study effects of total flavonoids from stems and leaves of Scutellaria baicalensis Georgi(SSF)on learning and memory deficits, automatic dyskinesia, neural and hepatic pathological changes and free radicals abnormal alterations.Methods Aluminum toxic model of mice was produced by introperitoneal injection (ip) of AlCl_3 for 50 d. Behavioral test of mice was used to examine the learning and memory ability;the number of automatic action determined the automatic dyskinesia;the neural and hepatic pathological changes were assessed by alterations of cerebral cortex and liver;MDA level and SOD activity in brain and liver were measured to evaluate free radicals.Results AlCl_3(100 mg·kg~-1 ,ip,50 d)resulted in a decreased ability of learning and memory in water maze task, lowered automatic action numbers, neuronal-hepatic-pathological changes and free radicals abnormal alterations, as compared with control group. The dose of SSF 50, 100 and 200 mg·kg~-1 significantly reversed the above pathological changes in toxic mice caused by aluminum. Conclusion SSF could reduce cognitive deficits and automatic dyskinesia, improve neuronal-hepatic-pathological changes and free radicals abnormal alterations.