PED subunit vaccine based on COE domain replacement of flagellin domain D3 improved specific humoral and mucosal immunity in mice |
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| Institution: | 1. State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou 510006, China;2. Guangdong Wenˊs Foodstuffs Group Co, Ltd, Yunfu 527300, China;1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China;1. College of Animal Science and Technology of Zhejiang A&F University, China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Lin’an, Zhejiang, PR China;2. School of Agricultural and Biological Engineer, Jinhua Polytechnic, Jinhua, Zhejiang, PR China;3. Zhejiang University Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, Zhejiang, PR China;1. Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA;2. Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota,1333 Gortner Avenue, St. Paul, MN 55108, United States;3. State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China;4. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China;1. Choongang Vaccine Laboratory, Daejeon 34055, Republic of Korea;2. Animal Virology Laboratory, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566, Republic of Korea;3. College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea;4. College of Veterinary Medicine, Jeju National University, Jeju 63243, Republic of Korea;5. HanByol Farm Tech, Namyangju, 12260, Republic of Korea;1. Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu 210095, PR China;2. Veterinary Medicine Research Centre of Beijing Da Bei Nong Group, Beijing 100195, PR China;1. Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, Jiangsu Province, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009, China |
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| Abstract: | Porcine epidemic diarrhea (PED) is an important re-emergent infectious disease and inflicts huge economic losses to the swine industry worldwide. To meet the pressing need of developing a safe and cost-efficient PED maternal vaccine, we generated three PED subunit vaccine candidates, using recombined Salmonella flagellin (rSF) as a mucosal molecular adjuvant. Domain D3 in rSF was replaced with COE domain of PEDV to generate rSF-COE-3D. COE fused to the flanking C′/N′ terminal of rSF yielded rSF-COE-C and rSF-COE-N. As a result, rSF-COE-3D could significantly improve COE specific antibody production including serum IgG, serum IgA, mucosal IgA and PEDV neutralizing antibody. Furthermore, rSF-COE-3D elicited more CD3+CD8+ T cell and cytokine production of IFN-γ and IL-4 in mouse splenocytes. In summary, our data showed that rSF-COE-3D could improve specific humoral and mucosal immunity in mice, thus suggesting that rSF-COE-3D could be applied as a novel efficient maternal PED vaccine. |
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| Keywords: | Porcine epidemic diarrhea COE Flagellin Subunit vaccine |
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