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The DNA repair gene PSO3 of Saccharomyces cerevisiae belongs to the RAD3 epistasis group
Authors:Mara S. Benfato  Martin Brendel  João A. P. Henriques
Affiliation:(1) Departamento de Biofisica, Instituto de Biociencias-UFRGS, 90049 Porto Alegre, RS, Brasil;(2) Centro de Biotecnologia, Instituto de Biociencias-UFRGS, 90049 Porto Alegre, RS, Brasil;(3) Institut für Mikrobiologie der J.W.-Goethe-Universität, Theodor-Stern-Kai 7, Haus 75, W-6000 Frankfurt am Main, Federal Republic of Germany
Abstract:Summary The mutant allele pso3-1 of Saccharomyces cerevisiae confers sensitivity to treatment with UV365nm (UVA) light-activated mono- and bi-functional psoralens. When pso3-1 is combined in double mutants with selected rad and pso mutant alleles and subjected to 8-MOP+UVA treatment, epistatic interaction with regard to survival is observed with pso1, pso2, and rad3. With the same treatment the combination of pso3-1 with rad6 and rad52 leads to synergistic interaction. For the monofunctional agent 3-carbethoxypsoralen (3-CPs) the analysis of double mutants yields the same results as with the bifunctional 8-methoxypsoralen (8-MOP) with the exception of the pso1-1pso3-1 double mutant. Here we find an additive interaction, i.e., the sensitivities of both parental strains are summed in the double mutant, which indicates a different substrate specificity of the repair activity encoded by the PSO1 and PSO3 genes.
Keywords:pso and rad mutants  Repair  S. cerevisiae  8-methoxypsoralen  3-carbethoxypsoralen
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