巨噬细胞对聚乙烯和钛合金颗粒吞噬反应的差异性

目的观察巨噬细胞对超高分子量聚乙烯(UHMWPE)颗粒和钛-6铝-4钒(Ti-6A l-4V)颗粒吞噬反应的差异。方法将巨噬细胞分别在含UHMWPE和Ti-6A l-4V颗粒的培养基中培养,在不同时段观察细胞吞噬颗粒的情况,并计算含有吞噬颗粒的巨噬细胞所占的比例。结果UHMWPE颗粒在培养后1 h即被巨噬细胞吞噬,且随时间延长,含有吞噬颗粒的巨噬细胞比例快速大量增加。而Ti-6A l-4V颗粒引起巨噬细胞吞噬现象出现的较晚,且在相同时段内含颗粒细胞的比例相对较小。结论UHMWPE颗粒能够引起巨噬细胞迅速而显著的吞噬反应,可能是其导致假体周围骨溶解的原因之一。

Different Phagocytosis Reaction of Marcophages on UHMWPE and Ti-6Al-4V Particles

Objective To investigate the difference in macrophages' phagocytosis of ultra-high molecular weight polyethylene(UHMWPE) particles and Ti-6Al-4V particles. Methods Macrophages were cultured in DMEM media with the above two types of particles respectively.The phagocytosis of the particles was observed at different times,and the ratio of cells containing particles against the sum total were calculated. Results UHMWPE particles were phagocytosed by macrophages within 1 h,and the ratio of cells containing particles rose rapidly.On the contrary,Ti-6Al-4V particles were phagocytosed much later,and the ratio of cells containing particles in Ti-6Al-4V group was lower than that in UHMWPE group at each corresponding time span. Conclusion UHMWPE particles can stimulate macrophages to phagocytose rapidly and significantly,which may lead to the induction of periprosthetic(osteolysis.)