砷剂对角质形成细胞CDK2、CDK4和Cyclin-E蛋白表达的影响

目的：研究砷剂对角质形成细胞细胞周期调控因子蛋白表达的影响。方法：以体外培养的角质形成细胞株为对象，一定浓度砷剂处理细胞后，用免疫印迹方法研究HaCaT细胞周期蛋白依赖激酶2（CDK2）、周期蛋白依赖激酶4（CDK4）和细胞周期蛋白E（Cyclin—E）的表达情况及砷剂对其表达的影响。结果：未受到砷剂处理的对照组细胞稳定表达CDK2、CDK4和Cyclin—E，当1nmol／L三氧化二砷处理24h后，HaCaT细胞表达CDK4、Cyclin—E水平升高，但CDK2的表达水平无明显变化。结论：一定浓度砷剂可刺激角质形成细胞增殖，可能与其升高细胞周期调控因子CDK4、Cyclin—E蛋白表达，导致S期细胞比例升高有关。

Effects of arsenic on CDK2, CDK4 and Cyclin-E expression in human epidermal keratinocytes

Objective: To determine the effect of arsenic on the expression of CDK2,CDK4 and cyclin-E in human epidermal keratinocytes. Methods: Human epidermal keratinocytes(cell line HaCaT) were cultured in vitro.After treated with certain concentration of arsenic,the expressions of CDK2,CDK4 and cyclin-E were measured by immunoblot test.Results: Comparing with control group,the expression of CDK4 and cyclin-E was increased significantly when cells were treated by 1 nmol/L arsenic trioxide for 24 h,while there was no increase in the expression of CDK2.(Conclusion): Certain concentration of arsenic may stimulate proliferation of HaCaT cell,which may be associated with increasing expression of CDK4 and cyclin-E,and the higher percentage cells in S phase.