HPLC法测定大鼠血浆中槐定碱

目的建立HPLC法测定大鼠血浆中槐定碱的体内分析方法,并用于其药动学研究。方法以苦参碱为内标,血浆样品在1.0mol·L-1氢氧化钠碱性条件下加入1.0mL三氯甲烷液-液萃取,采用Synergi 4μHydro-RP 80A(150mm×4.6mm,4.0μm)色谱柱分离,流动相为乙腈-磷酸盐缓冲溶液(0.01mol·L-1 KH2PO4,磷酸调pH 3.00)=5∶95,流速为1.0mL·min-1,柱温30℃,检测波长210nm,在此条件下测定。采用DAS 2.0软件进行药动学参数估算。结果槐定碱在0.25~25μg·mL-1内线性良好(r=0.999 7),提取回收率为76.6%~90.4%,日内、日间精密度符合要求。槐定碱主要药动学参数t1/2、AUC、Cmax分别为1.75±0.66h、10.80±2.69mg·h·L-1和5.56±0.78mg·L-1。结论该方法快速、灵敏、专属性强,可用于槐定碱药的代动力学分析。

Determination of the concentration of sophoridine in rat plasma by HPLC

Objective To establish a method for the determination of sophoridine in rat plasma by HPLC and to study its character‐istics of pharmacokinetics .Method Sophoridine and internal standard matrine were extracted from rat plasma with liquid‐liquid ex‐traction ,then were separated on a Synergi 4 μ Hydro‐RP 80A(150 mm × 4 .6 mm ,4 .0 μm) column with a mobile phase consisted of acetonitrile and phosphate buffer(pH 3 .00) (5∶95) at a flow rate of 1 .0 mL · min-1 .The detection wavelength was 210 nm , and the column temperature was 30 ℃ .The pharmacokinetic parameters were calculated with DAS 2 .0 practical pharmacokinetics program .Results The assay was linear in the range of 0 .25~25 μg · mL -1 ( r=0 .999 7) with recoveries ranged from 76 .6%to 90 .4% and satisfied inter‐and intra‐ day precision .The parameters of t1/2 ,AUC ,Cmax for sophoridine were 1 .75 ± 0 .66 h , 10 .80 ± 2 .69 mg · h · L -1 and 5 .56 ± 0 .78 mg · L -1 ,respectively .Conclusion T he developed method w as specific ,rapid and sen‐sitive ,and can be used for the determination of sophoridine in pharmacokinetic study .