功能性电刺激促进脑梗死大鼠肢体功能恢复的神经再生机制

目的:观察功能性电刺激(FES)对急性脑梗死大鼠神经功能和梗死侧室管膜下区(SVZ)神经干细胞(NSC)增殖的影响,探讨FES治疗脑梗死后神经功能恢复的可能机制.方法:将大脑中动脉阻塞(MCAO)模型大鼠随机分为FES治疗组、安慰电刺激组和假手术组,于造模后第2天开始进行FES治疗.采用改良神经功能损害评分(mNSS)评定大鼠神经功能,运用免疫荧光染色检测梗死侧SVZ区溴脱氧尿嘧啶核苷(BrdU)及BrdU/巢蛋白(nestin)、BrdU/神经胶质纤维酸性蛋白(GFAP)双标阳性细胞表达数量.结果:各时间点FES治疗组大鼠mNSS评分显著低于安慰电刺激组和假手术组(P＜0.05).治疗后FES治疗组大鼠脑梗死侧SVZ区BrdU+细胞数较安慰电刺激组和假手术组明显增加(P＜0.05),但各组BrdU+/nestin+细胞数占BrdU+细胞数比率无显著性差异(P＞0.05).结论:FES能显著促进脑梗死大鼠神经功能恢复,其中一个重要机制是通过促进梗死侧SVZ区NSC的增殖.

Study on effects of unctional electrical stimulation promoting neurologic function recovery and neural regeneration in rats with cerebral infarction

Abstract Objective: To investigate the effects of functional electrical stimulation(FES) on neurologic function of rats with acute cerebral infarction and to explore it's possible mechanism of proliferation of neural stem cells(NSC) in the ischemic subventricular zone(SVZ). Method: Acute cerebral infarction of rat's model was made by means of middle cerebral artery occlusion(MCAO). Rats were randomly allocated into FES group, placebo stimulation group and sham-operation group. FES treatment was carried out 2 days after operation. The modified neurological severity score(mNSS) was adopted to evaluate neurologic function. Bromodeoxyuridine(BrdU), BrdU /nestin and BrdU/glial fibrillary acid protein(GFAP) positive cells in the ischemic SVZ were detected by immunofluorescence staining technique. Result: The mNSS score in FES group was lower significantly than that in placebo stimulation group and sham-operation group(P<0.05). Compared with other two groups, the expression levels of BrdU+ cells in ischemic SVZ in FES group were significantly higher(P<0.05). The ratio of BrdU-nestin double-positive cells among BrdU-positive cells was not significantly different among three groups(P>0.05). Conclusion: FES treatment can significantly improve the recovery of neurologic function of rats with acute cerebral infarction. One of the important mechanisms was promoting NSCs proliferation in ischemic SVZ.