AB-8大孔吸附树脂纯化柴胡总皂苷工艺条件研究

目的 考察AB-8大孔吸附树脂对柴胡总皂苷的分离纯化方法.方法 根据柴胡总皂苷的结构特点,考察AB-8树脂的吸附性能,对AB-8吸附树脂纯化柴胡总皂苷的工艺条件进行了筛选,并采用紫外-可见分光光度法对柴胡总皂苷含量进行定量分析.结果 AB-8大孔吸附树脂对柴胡总皂苷具有较好的吸附选择性.吸附纯化的最佳工艺条件为吸附流速1BV/h,以70%乙醇作为洗脱剂,洗脱流速为2BV/h.采用上述工艺条件,产品纯度大于60%.结论 AB-8树脂用于柴胡总皂苷的分离纯化简单有效,易于工业化推广.

Separation and Purification of the Total Glucosides in Bupleurum Scorzonerifolium Willd. with AB-8 Macroporous Adsorption Resin

ObjectiveTo study the method for separating and purifying the total glucosides in Bupleurum scorzonerifolium Willd.with AB-8 macroporous adsorption resin. MethodsAccording to the structural characteristic of total glucosides, the resinAB-8 was systematically studied for its adsorption capability. The technological conditions of the adsorption resin AB-8 for separation was selected. UV-VIS was used to measure the content of total Glucosides. ResultsThe macroporous adsorption resin AB-8 can be used to produce the total glucosides with high quality. The optimal technological conditions were: the flow velocity of adsorption, 1BV/h; the eluting reagent, 70% ethanol; eluting velocity, 2BV/h. The purity of the total glucosides was over 60% with the technological conditions respectively. ConclusionIt is a simple and efficient method to separate the total glucosides from Bupleurum scorzonerifolium Willd. by usingAB-8 resin, and can be applied to manufacture.