| 血透患者血清对人脐静脉血管内皮细胞ICAM-1表达的影响 |
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| 引用本文： | 于文慧,郭学娜,孙桂江,于海波,董洪,刘益涛.血透患者血清对人脐静脉血管内皮细胞ICAM-1表达的影响[J].天津医药,2010,38(1). |
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| 作者姓名： | 于文慧 郭学娜 孙桂江 于海波 董洪 刘益涛 |
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| 作者单位： | 天津医科大学第二医院、天津市泌尿外科研究所、天津市泌尿外科重点实验室,300211 |
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| 摘 要： | 目的:研究维持性血液透析(MHD)患者血清对体外培养人脐静脉血管内皮细胞细胞间黏附分子(ICAM-1)表达的影响及其致内皮细胞功能紊乱的机制.方法:用10%胎牛血清1640培养液(对照组,A组)、10%正常人血清1640培养液(正常人组,B组)、10% MHD患者血清1640培养液(MHD患者组,C组)分别培养人脐静脉血管内皮细胞1、3、6、12 h,用免疫组织化学方法检测ICAM-1的表达情况.结果:不同血清刺激组人脐静脉血管内皮细胞 ICAM-1表达的差异有统计学意义(P < 0.05),与A组和B组比较,C组ICAM-1表达明显增加.不同刺激时间ICAM-1表达的差别有统计学意义(P < 0.05),不同血清刺激组ICAM-1的表达与刺激时间之间存在交互效应(P < 0.05).结论:慢性肾功能衰竭MHD患者血清可促进体外培养人脐静脉血管内皮细胞 ICAM-1的表达,从而导致内皮细胞功能失调.
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| 关 键 词： | 肾透析 血清 脐静脉 内皮细胞 细胞黏附分子 |
Effect of Serum from Maintenance Hemodialysis Patients on Expression of ICAM-1 in Human Umbilical Vein Endothelial Cells |
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| YU Wenhui,GUO Xuena,SUN Guijiang,YU Haibo,DONG Hong,LIU Yitao.Effect of Serum from Maintenance Hemodialysis Patients on Expression of ICAM-1 in Human Umbilical Vein Endothelial Cells[J].Tianjin Medical Journal,2010,38(1). |
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| Authors: | YU Wenhui GUO Xuena SUN Guijiang YU Haibo DONG Hong LIU Yitao |
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| Institution: | YU Wenhui,GUO Xuena,SUN Guijiang,YU Haibo,DONG Hong,LIU Yitao Second Hospital of Tianjin Medical University,Tianjin 300211,China |
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| Abstract: | Objective:To study the effect of serum from maintenance hemodialysis (MHD) patients on the expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVEC) cultured in vitro,and the mechanism of endothelial cells dysfunction caused by serum from MHD patients thereof. Methods:HUVEC were incubated for 1 h,3 h,6 h and 12 h in RPMI 1640 culture media containing 10 % fetal cattle serum (FCS )(group A),10% normal human serum (group B) and 10% MHD patient serum (group C) r... |
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| Keywords: | renal dialysis serum umbilical veins endothelial cells cell adhesion molecules |
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