抗骨增生片对兔膝骨关节炎软骨细胞bFGF及bFGF—mRNA的影响

目的：观察抗骨增生片对兔膝骨关节炎软骨细胞bFGF及bFGF-mRNA的影响，探讨抗骨增生片治疗骨关节炎是否与调节软骨细胞bFGF及bFGF-mRNA表达有关。方法：30只健康雄性日本大耳白兔随机分成正常组、模型组、抗骨增生片组。建立木瓜蛋白酶关节腔注射兔实验性膝骨关节炎模型，灌胃4周后，观察软骨细胞bFGF及bFGF-mRNA表达情况。以软骨细胞阳性细胞率、阳性细胞平均光密度、灰度值对观察目标量化。结果：抗骨增生片能上调软骨细胞bFGF及bFGF-mRNA的表达，抗骨增生片组与模型组和正常组比较差异均有统计学意义（P〈O．01），模型组和正常组比较差异无统计学意义（P〉O．05）。结论：抗骨增生片治疗膝骨关节炎可能与调节软骨细胞bFGF及bFGF-mRNA的表达有关。

Effects of Kanggu Zengsheng Tablets on bFGF Protein and mRNA Expression in Rabbit Chondrocytes with Knee Osteoarthritis

Objective：To explore the effects of Kanggu Zengsheng Tablets （KZT） on bFGF protein and mRNA expression in rabbit chondrocytes with knee osteoarthritis （KOA） and to investigate the mechanisms involved. Methods： A total of 30 healthy male Japanese rabbits were randomly divided into normal group, model group, and KZT group. After the knee osteoarthritis model was established by intra-articular injection of papain, respective treatments were given to each group by intragastric administration for 4 weeks. Then the expression of bFGF protein and mRNA was observed. Results：Both the protein and mRNA levels of bFGF were upregulated in KZT group compared to model group or normal group （P〈0.0l）. No significant difference was found between model group or normal group （P〉0.05）. Conclusion.. The clinical efficacy of KZT in knee osteoarthritis might be related to regulation of bFGF and bFGF mRNA expression in chondrocytes.