雷公藤对豚鼠睾丸附睾结构及功能的影响

本实验采用市售雷公藤多甙(GTW)灌喂豚鼠,6周后观察豚鼠睾丸和附睾的光镜结构及组织化学的变化,并应用Leitz显微分光光度计MPV-3扫描测定曲细精管初级精母细胞内DNA的相对含量,同时对附睾尾部精子作常规分析。结果表明:服药后曲细精管萎缩,管壁上皮变薄,细胞层次减少,精子细胞和精子极少或消失;曲细精管上皮细胞SDH及ACP酶活性减弱,ATP酶无明显变化;初级精母细胞核内DNA含量明显下降(P<0.01)。服药后附睾上皮细胞SDH活性减弱。ATP酶活性略为减弱,AKP无明显变化;附睾尾部精子的密度、活率及活力均显著下降(P<0.001)。结果提示:GTW对睾丸、附睾均有影响,对睾丸的作用更明显。

EFFECTS OF THE MULTIGLYCOSIDES OF TRIPTERYGIUM WILFORDII(GTW)ON THE STRUCTURE AND FUNCTION OF THE GUINEA-PIG TESTIS AND EPIDIDYMIS

GTW was given to 10 guinea-pigs by gastric gavage for 6 weeks. Then the animal testes and epididymides were removed for observation of the histological and histochemical changes, for examination of the epididymal spermatozoa and for measurment of the DNA contents of the primary spermatocytes. The results showed that in the treated guinea-pigs the seminiferous tubules were atrophied, with epithelium layers decreased, the number of spermatids and spermatozoa was drastically reduced. The activity of SDH and ACP in the testis and SDH and ATPase in the epididymis also decreased. The DNA ontents of primary spermatocytes significantly decreased(P<0.01). There was a marked reduction in the density, viability and motility of the epididymal spermatozoa(p<0.001). It suggests that GTW affected both the testis and the epididymis, and mainly damaged spermatocytes and spermatids.