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| RP-HPLC法同时测定人血清和脑脊液中苯巴比妥、苯妥英钠和卡马西平浓度 | |
| 引用本文： | 许贤瑞,董通,张庆,杨小英,党宏万. RP-HPLC法同时测定人血清和脑脊液中苯巴比妥、苯妥英钠和卡马西平浓度[J]. 宁夏医科大学学报, 2011, 0(4): 360-363 |
| 作者姓名： | 许贤瑞 董通 张庆 杨小英 党宏万 |
| 作者单位： | 宁夏医科大学;宁夏医科大学附属医院神经内科;宁夏医科大学附属医院药剂科; |
| 基金项目： | 宁夏自治区卫生厅重点科研项目(2009038); 宁夏自然科学基金重点项目(NZ101120) |
| 摘 要： | 目的建立RP-HPLC法同时测定人血清和脑脊液中苯巴比妥（PB）、苯妥英钠（PHT）和卡马西平（CBZ）浓度的方法。方法色谱柱：Agilent ZORBAX SB-C18（250mm×4.6mm,5μm）;流动相：甲醇-乙腈-水（27.5∶27.5∶45）;流速：1.0mL.m in-1;检测波长：210nm;柱温：40℃;进样量：10μL。结果血清中PB、PHT、CBZ的线性范围依次为：2.64～52.75μg.mL-1、1.45～29.00μg.mL-1、1.04～20.80μg.mL-1;脑脊液中PB、PHT、CBZ的线性范围依次为：1.32～42.20μg.mL-1、0.73～23.20μg.mL-1、0.52～16.64μg.mL-1;血清中PB、PHT、CBZ的平均提取回收率分别为：93.17%、96.17%、94.67%,脑脊液中PB、PHT、CBZ提取回收率接近100%;日间、日内RSD均小于4%。结论本方法简单、快速、准确,适用于临床血及脑脊液浓度的检测。 |
| 关 键 词： | 苯巴比妥 苯妥英 卡马西平 脑脊液 血清 高效液相色谱法 |
Simultaneous Determination of Phenobarbital,Phenytoin and Carbamazepine in Human Serum and Cerebrospinal Fluid by RP-HPLC |
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| XU Xian-rui,DONG Tong,ZHANG Qing,YANG Xiao-ying,DANG Hong-wan. Simultaneous Determination of Phenobarbital,Phenytoin and Carbamazepine in Human Serum and Cerebrospinal Fluid by RP-HPLC[J]. Journal of Ningxia Medical College, 2011, 0(4): 360-363 | |
| Authors: | XU Xian-rui DONG Tong ZHANG Qing YANG Xiao-ying DANG Hong-wan |
| Affiliation: | XU Xian-rui1,DONG Tong1,ZHANG Qing2,YANG Xiao-ying3,DANG Hong-wan3(1.Ningxia Med.Univ.,2.Dept.of Neurology,the Affiliated Hospital of Ningxia Med.Univ.,3.Dept.of Pharmacy,the Affiliated Hospital of Ningxia Med.Univ.Yinchuan,750004) |
| Abstract: | Objective To establish RP-HPLC method for simultaneous determination of phenobarbital（PB）,phenytoin（PHT） and carbamazepine（CBZ） in human serum and cerebrospinal fluid（CSF）.Methods Aglient ZORBAX SB-C18（250mm×4.6mm,5μm） was used.The mobile phase was water-methanol-acetonitrile（27.5∶27.5∶45）.The flow rate was 1.0 mL·min-1 and the detection wavelength was 210nm.The column temperature was 40℃ and sample size was 10μL.Results The linear relation of PB,PT and CBZ were 2.64～52.75μg·mL-1,1.45～29.00μg·mL-1,1.04～20.80μg·mL-1 respectively in human serum and 1.32～42.20μg·mL-1,0.73～23.20μg·mL-1,0.52～16.64μg·mL-1 in cerebrospinal fluid respectively.The average recovery rate of PB,PHT and CBZ were 93.17%、96.17%、94.67% respectively in human serum and nearly 100% in cerebrospinal fluid.The RSD for the inter-day and intra-day of three drugs were less than 4%.Conclusion The method is sample,fast,accurate and suitable for determination PB,PHT and CBZ in human serum and cerebrospinal fluid of drug concentration. |
| Keywords: | phenobarbital phenytoin carbamazepine cerebrospinal fluid serum HPLC |
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