RP-HPLC测定降香煎剂中橙花叔醇含量及变化

目的:建立对降香中橙花叔醇含量随煎煮时间变化的分析方法。方法:采用高效液相色谱法,以甲醇:水为流动相(78:22),检测波长:213nm,体积流速:1.2ml/min,柱温:室温,检测降香沸水后下时每间隔1 min所取水煎液样品中橙花叔醇的含量。结果:在煮沸后20min内,橙花叔醇含量呈上升趋势,在2.8min时达到相对高点;之后,呈下降趋势。结论:沸腾后仍用文火继续煎煮约2.8min时降香中的橙花叔醇的含量相对较高。

To determine nerolidol content and variations in dalbergia odorifera decoction with RP-HPLC

Objective：To explore methods to analyze how nerolidol contents vary with boiling time of dalbergia decoction. Method：HPLC was used, with the mobile phase consisted of methanol-water （78：22）, determine wavelength of 213nm, volumetric flow rate of 1.2ml/min, and column temperature of room temperature. We tested nerolidol contents sampled from dalbergia decoction at 1min intervals after boiled. Results：During the 20 minutes after the decoction boiled, the contents took on an ascending tendency;afterwards, it took on a descending tendency. Conclusion：The highest content was obtained at 2.8min after the decoction boiled.