陕西省HIV-1流行株膜蛋白基因 C2-V3区序列特征和亚型分析

目的了解陕西省HIV-1毒株的流行情况和亚型特征.方法用套式聚合酶链式反应(Nested-PCR)对6份采集于陕西省经确认为HIV-1感染者或艾滋病病人外周血淋巴细胞(PBMC)提取核酸,从6份样品中获得了HIV-1膜蛋白(ENV)基因的核酸片段进行扩增,并测定和分析了C2-V3及其邻区共312个核苷酸序列.结果 6份血样中,2份为HIV-1A亚型毒株感染(SHX7、SHX8);4份为HIV-1B'亚型(泰国B'亚型)毒株感染(SHX1、SHX5、SHX6、SHX9).SHX1、SHX5、SHX6、SHX9彼此间基因离散率为5.62%,SHX7与来自卢旺达的U08794之间的基因离散率仅为2.41%.结论陕西省目前存在HIV-1 A、B'两种亚型的毒株,HIV-1 B'亚型由邻近地区传入,HIV-1 A亚型为与非洲人接触传入.

Subtype and Sequence Analysis of the C2 - V3 Region of gp120 Gene among HIV - 1 Strains in Shanxi Province

Objective To study the epidemic situation and characteristics of subtypes of HIV-1 strains prevalent in Shanxi Province. Methods Six DNA fragments of HIV-1 ENV gene were amplified by nested PCR from uncultured peripheral blood mononuclear cells(PBMCs)obtained from six HIV- 1 seropositive individuals or AIDS patients identified in Shanxi Province.The C2 - V3 region (about 450bp) was sequenced.Results There were two strains of HIV-1 subtype A and four strains of HIV-1 subtype B', and the gene divergence of B' subtype was 5.62% .The 2 strains of HIV-1 subtype A were closely related to the U08794 from Rwanda, while the 4 strains of HIV - 1 subtypes B' were closest to those of subtype B in Thailand.Conclusion The study and epidemiological data showed that strains of HIV-1 subtype A and B' were prevailing in Shanxi Province.