|
|||||
|
|
| 诊断用彩色多普勒超声辐照对小鼠早孕胚胎细胞凋亡的影响 | |
| 引用本文： | 李秀琴,沈燕琼,冀春萱. 诊断用彩色多普勒超声辐照对小鼠早孕胚胎细胞凋亡的影响[J]. 临床医药实践, 2009, 18(1) |
| 作者姓名： | 李秀琴 沈燕琼 冀春萱 |
| 作者单位： | 1. 山西医科大学,山西,太原,030001;太原市中心医院,山西,太原,030009 2. 山西省职工医学院,山西,太原,030012 3. 山西医科大学,山西,太原,030001 |
| 摘 要： | 目的:探讨诊断用彩色多普勒超声对小鼠早孕胚胎组织细胞凋亡的影响。方法:20只孕6~7 d成年昆明种小鼠,按彩超辐照时间不同随机等分为4组,Ⅰ组(对照组)、Ⅱ组(辐照10 min组)、Ⅲ组(辐照20 min组)、Ⅳ组(辐照30min组),仪器采用百胜公司DU-8型彩色超声诊断仪。照射条件:腹部凸阵探头,探头频率为3.0MH z,M I=0.9,T is=0.9,照射后24 h取小鼠胚胎标本。利用流式细胞仪检测凋亡细胞百分率。结果:各组胚胎组织均存在凋亡细胞,照射30 min组凋亡细胞百分率高于0 min组,差异有显著性(P<0.05)。结论:诊断用彩色多普勒超声过度辐照早孕小鼠,可诱发胚胎组织细胞凋亡。 |
| 关 键 词： | 超声 凋亡 胚胎 |
Effect of diagnostic color Doppler ultrasound on apoptosis in rat embryonic cells |
|
| LI Xiu-qin,SHEN Yan-qiong,JI Chun-xuan. Effect of diagnostic color Doppler ultrasound on apoptosis in rat embryonic cells[J]. Proceeding of Clinical Medicine, 2009, 18(1) | |
| Authors: | LI Xiu-qin SHEN Yan-qiong JI Chun-xuan |
| Abstract: | Objective: To investigate changes of apoptosis in rat embryonic cells induced by diagnostic color Doppler ultrasound.Methods:Early pregnant rats were irradiated with diagnostic dosages of color Doppler ultrasound.Twenty rats were randomly devid edinto four groups according to different exposure time:groupo min,grouplo min,group 20 min,group 30min.The pecentage of apoptosis in rat embryos was detected by flow cytometry(Annexin V/PI).Results:The percentage of apoptosis in the embryos in group 30 min was higher than that in groupo min(P<0.05).Conclusion:Continuous irradiation by diagnostic dosages of color Doppler ultrasound may induce embryonic cells apoptosis. |
| Keywords: | ultrasound apoptosis embryo |
| 本文献已被 维普 万方数据 等数据库收录！ | |