The Ultrastructure of MCF-7 Breast Cancer Cells after Vasodilator-Stimulated Phosphoprotein Knockdown |
| |
| Authors: | Ying Wang Ke Su Peng-Chao Hu Fang-Fang Gao Jing-Wei Zhang |
| |
| Affiliation: | 1. Department of Pathology and Pathophysiology, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University School of Basic Medical Sciences, Wuhan, Hubei, China,;2. Division of Nephrology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China, and;3. Department of Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan University, Wuhan, Hubei, China |
| |
| Abstract: | ![]()
Inhibition of vasodilator-stimulated phosphoprotein (VASP) expression could modulate the adhesion and proliferation of breast cancer cells. However, the underlying mechanisms are not well defined. Here, we show that knockdown of the VASP changes the ultrastructure of human MCF-7 breast cancer cells. Transfection of VASP shRNA significantly lowered the expression of VASP protein in MCF-7 cells. In the shRNA-VASP group, immunofluorescence showed diminished presence of F-actin, and it was lower in the nucleus than in the cytoplasm. After VASP was inhibited, the MCF-7 cells were oval in shape with blunt lamellipodium, disappearance of the cristae of mitochondria, decreased microvilli and more vacuoles. Collectively, our findings elucidated the morphological mechanism that knockdown of the VASP changed the ultrastructure of MCF-7 cells. |
| |
| Keywords: | Knockdown MCF-7 ultrastructure changes VASP |
|
|
